孕早期苯并（a）芘暴露对小鼠胚胎着床的影响

沈茶; 何俊琳; 刘学庆; 丁裕斌; 高茹菲; 王应雄; 陈雪梅

doi:10.13406/j.cnki.cyxb.000954

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孕早期苯并（a）芘暴露对小鼠胚胎着床的影响

生殖系统疾病 | 更新时间：2024-01-17

- 孕早期苯并（a）芘暴露对小鼠胚胎着床的影响
- Effect of Benzo(a)pyrene on embryo implantation in mice during early pregnancy
- 重庆医科大学学报 2016年41卷第7期页码：675-679
- 作者机构：
  
  重庆医科大学公共卫生与管理学院生殖生物学教研室
- 作者简介：
- 基金信息：
  
  国家自然科学基金资助项目（编号:81202234、81573175）;重庆市科学技术委员会资助项目（编号:cstc2013jcyjA10046）
- DOI：10.13406/j.cnki.cyxb.000954
  中图分类号： R714.2
- 纸质出版日期：2016，
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[1]沈茶,何俊琳,刘学庆,丁裕斌,高茹菲,王应雄,陈雪梅.孕早期苯并(a)芘暴露对小鼠胚胎着床的影响[J].重庆医科大学学报,2016,41(07):675-679.

Shen Cha, He Junlin, Liu Xueqing, et al. Effect of Benzo(a)pyrene on embryo implantation in mice during early pregnancy[J]. Journal of Chongqing Medical University, 2016,41(7):675-679.
[1]沈茶,何俊琳,刘学庆,丁裕斌,高茹菲,王应雄,陈雪梅.孕早期苯并(a)芘暴露对小鼠胚胎着床的影响[J].重庆医科大学学报,2016,41(07):675-679. DOI： 10.13406/j.cnki.cyxb.000954.

Shen Cha, He Junlin, Liu Xueqing, et al. Effect of Benzo(a)pyrene on embryo implantation in mice during early pregnancy[J]. Journal of Chongqing Medical University, 2016,41(7):675-679. DOI： 10.13406/j.cnki.cyxb.000954.

摘要

目的:探讨孕早期Ba P暴露对胚胎着床的影响

为进一步完善Ba P对雌性生殖功能的影响提供实验依据。方法:将60只孕鼠随机分为对照组和Ba P组

每组30只

每组再随机分成3小组

每组10只。从妊娠第1天对照组用玉米油、Ba P组用0.2 mg/（kg·d）剂量Ba P进行灌胃染毒

至妊娠的第4天（D4）、第5天（D5）、第6天（D6）处死并取材。计数小鼠胚胎着床点数目

ELISA方法检测小鼠血浆中雌激素和孕激素水平

荧光定量PCR、Western blot及免疫组织化学方法检测小鼠子宫内膜中雌激素受体-α（estrogen receptor-α

ERα）和孕激素受体（progesterone receptor

PR）水平。结果:比较对照组与Ba P组的胚胎着床数

Ba P组的着床点数在妊娠D5和D6均低于对照组

差异有统计学意义（P<0.05）;Ba P组血浆雌激素水平和孕激素水平均高于对照组

差异均有统计学意义（P<0.05）

不同染毒时间的雌激素水平与孕激素水平不同

由高到低依次为妊娠D6、D5、D4

各时间点的差异均有统计学意义（P<0.05）;ERα的m RNA水平在Ba P组的表达高于对照组

差异有统计学意义（P<0.05）

不同染毒时间ERα的m RNA水平不同

妊娠D6的表达高于妊娠D4和D5

差异有统计学意义（P<0.05）

PR的m RNA水平在Ba P组的表达低于对照组

差异有统计学意义（P<0.05）。Western blot和免疫组化结果也表明苯并（a）芘暴露组小鼠子宫中ERα表达量升高

PR表达量下降。Western blot和免疫组化结果也表明Ba P组小鼠子宫中ERα表达量升高

PR表达量下降。结论:Ba P可能通过影响孕鼠雌孕激素水平及PR的表达模式

最终导致胚胎着床点数的下降。

Abstract

Objective:To illuminate the effects of benzo(a)pyrene(Ba P)on embryo implantation during the early pregnancy. Methods:Totally 60 pregnant mice were randomly divided into two groups. The control group was administered corn oil only

and the treated groups were exposed to Ba P at 0.2 mg/kg body weight by oral gavage once a day. Pregnant mice were killed on days 4-6 by cervical dislocation

10 mice each day. The uteri tissues were collected. The numbers of the implantation sites were calculated. The levels of estradiol(E2)and progesterone(P4)in plasma were determined by ELISA. q RT-PCR and Western blot were employed to detect the expression of the estrogen receptor-α(ERα)and the expression of progesterone receptor(PR). Results:Compared with those of control group

the numbers of embryo implantation sites were decreased in the Ba P groups(P<0.05). The result of ELISA indicated that the levels of E2 and P4 in plasma were elevated in Ba P treatment groups(P<0.05). q RT-PCR showed that the m RNA level of ERαwas up-regulated(P<0.05)and m RNA level of PR was down-regulated in the Ba P treatment groups(P<0.05). Western blot and IHC showed that expression of ERα was up-regulated while the expression of PR was down-regulated after Ba P exposure. Conclusion:Ba P can cause the disorder of ERα and PR in the process of embryo implantation in mice

which will affect the expression pattern of ERα and PR and lead to the decrease in the number of embryo implantation.

关键词

苯并（a）芘雌孕激素胚胎着床小鼠

Keywords

benzo(a)pyreneestradiol and progesteroneembryo implantationmice

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