Objective:To investigate the effect of glycyrrhizin on atopic dermatitis in mice model.Methods:A model of atopic dermatitis was established in 6-8-week-old female BALB/c mice by applying 2

4-dinitrochlorobenzene（DNCB）to shaved dorsal skin.During the process of establishment of model

the mice were divided into four groups:control group

three glycyrrhizin groups treated with50 mg/（kg·d）

20 mg/（kg·d）and 10 mg/（kg·d）

respectively.The treatment lasted for 14 days.On day 8 and 15

dorsal skin tissue samples were obtained from these mice and for the observation of histopathological changes by using hematoxylin and eosin（HE）staining.The mRNA levels of Th2 cytokines were evaluated by qRT-PCR.Enzyme linked immunosorbent assay（ELISA）was carried out to determine the levels of IL-4 and IL-13

secreted by spleen lymphocytes followed by stimulation of Con A.Results:Compared with the control mice

those treated with different doses of glycyrrhizin showed no difference in pathological changes of skin tissue and mRNA levels and cytokine production of IL-4 and IL-13 on day 8 after the treatment.However

on day 15

dermal and epidermal thickness of skin lesions（141.00±7.61）vs.（194.00±18.43）;（281.20±69.42）vs.（374.70±40.01）（P=0.005

P=0.012

respectively）as well as the mRNA expression of IL-4（0.036±0.011 vs.0.073±0.007

respectively）and IL-13（0.051±0.022 vs.0.100±0.015）（P=0.000

P=0.001

respectively）and the levels of IL-4（42.94±9.26 vs.61.87±10.20）and IL-13（43.34±12.59）vs.（77.42±10.71）（P=0.002

P=0.000

respectively）secreted by spleen lymphocytes from mice treated with glycyrrhizin 50 mg/（kg·d）group showed a significantly decrease compared with those of control mice.Conclusion:Glycyrrhizin can inhibit the inflammation in mice model of atopic dermatitis

possibly by down regulating the expression cytokines of type T helper2 cells.