Objective:To study the pharmacokinetic characteristics and bio-equivalence of evodiamine nanoemulsion（EDN）prepared by the experiment in rats. Methods:Twelve male SD rats were randomly divided into 2 groups

and single dose of evodiamine（ED）or EDN was administrated respectively in the experiment. The blood was took from eye socket at 0

5

15

30

45 min and at 1

2

5

8

12

24

48

72 h after administration. The plasma concentration of ED was determined by HPLC;pharmacokinetic parameters was analyzed by DAS software;the bio-equivalence between ED and EDN was evaluated by analysis of variance and two one-sided test.Results:The main pharmacokinetic parameters of EDN:area under concentration-time curve（AUC（0

7

2 h））

peak concentration（Cmax）and peak time（Tmax）were（3 636.79±1 642.94）μg/L·h

（192.49±37.68）μg/L

（4.94±1.67）h

respectively. The oral bio-availability of EDN in rats was about 4.66 times higher than that of ED. The 90% confidence interval of AUC（0

7

2 h）exceeded the bio-equivalence criteria interval. Conclusion:EDN oil-in-water is successfully prepared. The bio-availability of ED in rats increases remarkably after oral administration of EDN.