Objective:To investigate the effects of autophagy related protein Atg5 and P62 on the process of newborn mice follicular dysplasia caused by DEHP. Methods:Adult female Kun Ming mice of six to eight weeks at clean and healthy level were randomly divided into the control groups(corn oil)and the DEHP groups(30 in each group)without any treatment. The newborns were divided into the control groups(corn oil)and the DEHP groups. By intraperitoneal injection

the newborns of the DEHP groups were injected with DEHP at the concentration of 0.62 mg/kg

and the ones of the control groups were injected with the same volume of corn oil.The newborn mice were injected once a day from born to 5 days postpartum. Ovarian tissues of the newborn mice from two groups were collected. HE staining was used to observe the development of ovarian follicles at all levels. Real-time PCR and Western blot were employed to detect the expression of follicular developmental marker GDF9 and autophagy related proteins Atg5 and P62.Results:Compared with that of control groups

the number of primordial follicles of newborn mice from the DEHP groups decreased sig-nificantly

and there was no significant difference in the primary follicles number of the newborn mice between the two groups. The re-sults of Real-time PCR showed the levels of follicular developmental marker GDF9 decreased significantly

butautophagy related gene Atg5 and P62 m RNA in the newborn mice ovarian tissues from the DEHP group increased significantly. Western blot showed the expression of autophagy related proteins Atg5 and P62 in the newborn mice ovarian tissues were significantly increased following DEHP treatment. Conclusion:Atg5 and P62 might play an important role during the follicular dysplasia process of newborn mice induced by DEHP.