Objective:To observe the changes of β-dystroglycan(β-DG)expression in the brain post intracerebral hemorrhage(ICH)

and to explore its effect on the polarized expression of aquaporin4(AQP4). Methods:Sixty-eight male SD rats were randomly divided into sham group and ICH group(including ICH post day 1

day 3

and day 7 groups). Autologous blood was injected into the right caudate nucleus to establish ICH model in rats. HE staining and transmission electron microscope(TEM)were used to observe the structure changes;Western blot was used to detect the changes of β-DG protein in the perihematomal tissue and the co-expression of AQP4 and β-DG were detected by immunofluorescence. Results:In the perihematomal tissue

the organization structure was destroyed obviously

especially in the tissue of ICH post day 1 group;the endothelial cell of capillary was swollen and perivasicular gap was widened. In the perihematomal tissue

the β-DG protein of ICH post day 1

day 3

day 7 groups was(0.251±0.038)

(0.396±0.069)

(0.398±0.086)respectively

signifantly different from that of(0.610 ± 0.121)in sham group(P=0.000). Different from the co-expression of AQP4 and β-DG in the sham group

the expression of β-DG was significantly decreased in the brain tissue post ICH

while the polarized expression of AQP4 was lost. Conclusion:The decreased expression of β-DG may contribute to the loss of AQP4 polarized expression in the brain tissue post ICH.