Objective:To investigate the mechanism of Danshen drug-containing serum on transforming growth factor β1(TGF-β1)and CyclinD1 in hepatic stellate cells. Methods: According to the random number table

20 Sprague-Dawley(SD)rats were randomly divided into the Danshen(1.04 g/kg

2.08 g/kg

and 4.17 g/kg dosage)groups and normal control group. Blood was collected after gastric perfusion at 2 hours on 10 th day. The method of high performance liquid chromatography(HPLC)was used to detect the composition of Danshen in serum. HSCs were cultured in vitro and stimulated with platelet-derived growth factor-BB(PDGF-BB)

then were treated separately with the serum of Danshen(high

middle

and low dosage)

normal saline for 24 hours. After cells being collected

the expressions of retinoic acid receptor and TGF-β1 mRNA were measured by real-time PCR(q RT-PCR)

the content of α-smooth muscle actin(α-SMA)

typeⅠcollagen and CyclinD1 were detected by Western blot. Results:Compared with the normal control group

Danshen(low

middle

and high)groups could increase the expressions of retinoic acid receptor mRNA

and decrease the contents of TGF-β1 mRNA

α-SMA

type Ⅰ collagen and CyclinD1. Conclusion:Danshen may be achieved through increasing the expression of retinoic acid receptor and reducing TGF-β1 and CyclinD1 to inhibit the proliferation of HSCs

thus play a role of inhibition in liver fibrosis.