P2X3受体对大鼠海马CA1区锥体神经元兴奋性的影响

周鑫; 陈阳美

doi:10.13406/j.cnki.cyxb.000855

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P2X3受体对大鼠海马CA1区锥体神经元兴奋性的影响

神经病学 | 更新时间：2024-01-17

- P2X3受体对大鼠海马CA1区锥体神经元兴奋性的影响
- Effects of P2X3 receptor on neuronal excitability in hippocampal CA1 pyramidal cells of rats
- 重庆医科大学学报 2016年41卷第1期页码：14-17
- 作者机构：
  
  重庆医科大学附属第二医院神经内科
- 作者简介：
- 基金信息：
  
  国家自然科学基金资助项目（编号:81171225）
- DOI：10.13406/j.cnki.cyxb.000855
  中图分类号： R741
- 纸质出版日期：2016，
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[1]周鑫,陈阳美.P2X3受体对大鼠海马CA1区锥体神经元兴奋性的影响[J].重庆医科大学学报,2016,41(01):14-17.

Zhou Xin, Chen Yangmei. Effects of P2X3 receptor on neuronal excitability in hippocampal CA1 pyramidal cells of rats[J]. Journal of Chongqing Medical University, 2016,41(1):14-17.
[1]周鑫,陈阳美.P2X3受体对大鼠海马CA1区锥体神经元兴奋性的影响[J].重庆医科大学学报,2016,41(01):14-17. DOI： 10.13406/j.cnki.cyxb.000855.

Zhou Xin, Chen Yangmei. Effects of P2X3 receptor on neuronal excitability in hippocampal CA1 pyramidal cells of rats[J]. Journal of Chongqing Medical University, 2016,41(1):14-17. DOI： 10.13406/j.cnki.cyxb.000855.

摘要

目的:研究P2X3受体（P2X3 receptor

P2X3R）在海马CA1区的定位及对CA1区锥体细胞兴奋性的影响。方法:免疫荧光技术对P2X3R在海马CA1区表达进行定位

SD鼠麻醉后断头取脑

修块后应用振动切片机切出350μm脑片并用正常人工脑脊液26℃恒温孵育1 h

通过膜片钳全细胞记录技术

给予P2X3R选择性拮抗剂AF-353和非选择性激动剂α

β-Me ATP干预

记录锥体细胞动作电位发放频率的改变。结果:P2X3R在海马区主要表达于神经元上

在星形胶质细胞不表达。P2X3R拮抗剂AF-353减慢锥体细胞动作电位发放频率减小其兴奋性[（0.788±0.163）Hz vs.（0.352±0.079）Hz

t=7.32

P=0.002]

β-Me ATP则加快动作电位发放频率[（0.715±0.186）Hz vs.（1.610±0.454）Hz

t=-4.97

P=0.008]

增加兴奋性。结论 :P2X3R在海马区主要表达于神经元树突及胞质并且影响神经元兴奋性

可能参与了兴奋性增高有关疾病的发生发展过程。

Abstract

Objective:To investigate the effects of P2X3 receptor(P2X3R)on neuronal excitability in hippocampal CA1 pyramidal cells of rats. Methods:The localization of P2X3 R was detected by immunofluorescence. Sprague-Dawle rats were euthanized via decapitation after anesthetization and the brain tissues were trimmed and placed in a vibratome to obtain a slice thickness of 350 μm.The slices then were incubated in normal artificial cerebrospinal fluid at 26 ℃ for 1 h. Intervention of AF-353 and α

β-Me ATP was performed and the action potentials(APs)frequency was recorded via whole cell patch-clamp technique. Results:The P2X3 R was located at the neuron instead of the astrocyte in hippocampal CA1 region. The antagonist of AF-353 decelerated the APs frequency and reduced cellular excitability[(0.788±0.163)Hz vs.(0.352±0.079)Hz

t=7.32

P=0.002] whereas α

β-Me ATP agonist had the reverse effects[(0.715±0.186)Hz vs.(1.610±0.454)Hz

t=-4.97

P=0.008]. Conclusion:P2X3R effect neuronal excitability and may participate in the process and development of diseases related to hyperexcitability.

关键词

P2X3受体兴奋性癫痫阿尔茨海默病

Keywords

P2X3R receptorexcitabilityepilepsyAlzheimer’s disease

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