Objective:To understand the function of ovarian hormone synthesis change through the analysis of the change of hyperlipidemia and granulosa cells. Methods:Rats under high-fat were fed to sexual mature. Ovarian granulosa cells were observed by transmission electron microscope

and sex hormone receptors and growth differentiation factor-9(GDF-9)changes in ovarian granulosa cells were detected by immunohistochemistry and Western blot. Results:After high-fat diet

granule cell pathological changes being found;estrogen

progesterone and androgen receptors levels were reduced obviously;the expression of GDF-9 in ovarian tissues was weakened;ovarian cell function was declined. Conclusion:After high-fat fed

the damage change of granular cell structure and the decline of hormone synthesis function are consistent with the clinical symptoms of weakened ovulation under hyperlipemia

suggesting the role of hyperlipidemia for weaken ovarian ovulation function by injuring granular cell.