Objective:To characterize the expression of G-protein coupled receptor 30(GPR30)in trophoblast cells at different conditions

and to determine the effect of disordered expression of GPR30 on the development of preeclampsia(PE). Methods:The expressions of GPR30 protein were detected not only in villi at first trimester

placentas from normal term and pregnancies complicated with PE

but also in HTR8/SVneo cells which treated with normoxia and hypoxia-reoxygenation(H/R)by immunofluorescence

immunohistochemistry and/or Western blot. The pharmacological modulations of GPR30 were built by the using of 17 β-estradiol(E2)

GPR30 specific agonist G1 and GPR30 specific inhibitor G15. H/R was applied to simulate the pathological condition of PE;their effect on human trophoblast cell invasion was determined using vitro invasion assays. Results:GPR30 protein was highly expressed in the cytotrophoblast of first trimester villi and the term placentas from the gestational matched controls(GMCs)

but significantly compromised in PE cells model and placentas which complicated by PE. In addition

E2 and G1 treatment significantly increased the invasive capacity of trophoblast cells

however

the antagonist G15 play an opposite role. Meanwhile

H/R significantly attenuated invasiveness of trophoblast cells. Conclusion:the pathogenetic mechanism of PE is associated with the downregultaion of GPR30 protein level in trophoblast which may impair the invasive capacity of trophoblast cells.