载天门冬酰胺酶自组装透明质酸-聚乙二醇/γ-环糊精纳米囊的体外活性和稳定性探究

李瑶; 晏子俊; 李万玉; 胡雪原; 何丹; 张景勍

doi:10.13406/j.cnki.cyxb.000789

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载天门冬酰胺酶自组装透明质酸-聚乙二醇/γ-环糊精纳米囊的体外活性和稳定性探究

技术方法 | 更新时间：2024-01-17

- 载天门冬酰胺酶自组装透明质酸-聚乙二醇/γ-环糊精纳米囊的体外活性和稳定性探究
- Study on in vitro activity and stability of asparaginase loaded in self-assembly of polyethylene glycol-hyaluronic acid/γ-cyclodextrin nanocapsules
- 重庆医科大学学报 2016年41卷第5期页码：456-460
- 作者机构：
  
  重庆医科大学药物高校工程研究中心
- 作者简介：
- 基金信息：
  
  国家自然科学基金资助项目（编号:30973645）;重庆市教委资助项目(高校优秀人才资助项目)
- DOI：10.13406/j.cnki.cyxb.000789
  中图分类号： R943
- 纸质出版日期：2016，
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[1]李瑶,晏子俊,李万玉,胡雪原,何丹,张景勍.载天门冬酰胺酶自组装透明质酸-聚乙二醇/γ-环糊精纳米囊的体外活性和稳定性探究[J].重庆医科大学学报,2016,41(05):456-460.

Li Yao, Yan Zijun, Wanyu Li, et al. Study on in vitro activity and stability of asparaginase loaded in self-assembly of polyethylene glycol-hyaluronic acid/γ-cyclodextrin nanocapsules[J]. Journal of Chongqing Medical University, 2016,41(5):456-460.
[1]李瑶,晏子俊,李万玉,胡雪原,何丹,张景勍.载天门冬酰胺酶自组装透明质酸-聚乙二醇/γ-环糊精纳米囊的体外活性和稳定性探究[J].重庆医科大学学报,2016,41(05):456-460. DOI： 10.13406/j.cnki.cyxb.000789.

Li Yao, Yan Zijun, Wanyu Li, et al. Study on in vitro activity and stability of asparaginase loaded in self-assembly of polyethylene glycol-hyaluronic acid/γ-cyclodextrin nanocapsules[J]. Journal of Chongqing Medical University, 2016,41(5):456-460. DOI： 10.13406/j.cnki.cyxb.000789.

摘要

目的:制备载天门冬酰胺酶（asparaginase

AN）自组装透明质酸-聚乙二醇（hyaluronic acid-graft-poly（ethylene glycol）

HA-g-PEG）/γ-环糊精（γ-cyclodextrin

γ-CD）纳米囊（HA-g-PEG/γ-CD nanocapsules loaded with AN

AHRPs）

并对AHRPs及游离AN在体外的活性及稳定性进行研究。方法:采用自组装法制备了AHRPs

考察了AHRPs的透射电镜、粒径、Zeta电位、最适温度和最适p H

并通过贮存稳定性、抗胰蛋白酶水解能力、抗部分金属离子及有机化合物、热稳定性、酸碱稳定性和血浆稳定性等实验

对游离AN与AHRPs体外稳定性差异进行了一定的考察。结果:AHRPs的粒径为（410.30±3.20）nm

Zeta电位为（-31.40±1.65）m V

AHRPs和游离AN的最适温度分别为50℃和60℃

最适p H值均为7.5。稳定性实验结果显示

相同条件下

AHRPs的贮存稳定性、抗胰蛋白酶水解能力、抗金属离子和有机化合物、热稳定性、酸碱稳定性和血浆稳定性均优于游离AN。结论:AHRPs能同时提高游离AN在体外的活性及稳定性。

Abstract

Objective:To prepare asparaginase(AN)loaded in self-assembly of polyethylene glycol-hyaluronic acid/γ-cyclodextrin nanocopsules(AHRPs)

and then to investigate the in vitro activity and stability difference between AHRPs and free AN. Methods:AHRPs was prepared by self-assembly method. The image of AHRPs under the transmission electron microscopy was observed. The size

zeta potential

optimal temperature and optimal p H value were detected. The in vitro activity and stability of AHRPs and free AN were investigated by measuring storage stability

trypsin stability

the effect of several metal ions and organic compounds

thermal stability

acid- and basic-stabilities and plasma stability on free AN and AHRPs. Results:After calculating

the particle size was(410.30±3.20)nm

zeta potential was(-31.40±1.65)m V. The optimum temperature of AHRPs and free AN was 50 ℃ and 60 ℃

respectively. The optimum p H of AHRPs and free AN was 7.5. The results of stability experiments showed that

storage stability

trypsin stability

the effect of several metal ions and organic compounds

thermal stability

acid- and basic-stabilities and plasma stability of AHRPs all were better than those of free AN. Conclusion:AHRPs can improve the activity and significantly enhance the stability of AN in vitro.

关键词

天门冬酰胺酶空心纳米囊稳定性最适p H最适温度

Keywords

asparaginasehollow nanocapsulesstabilityoptimal p Hoptimal temperature

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