Objective:To investigate the prevention and treatment effects and to preliminary discuss the action mechanisms of lycium barbarum polysaccharide(LBP)on hepatocyte steatosis model of hepatocyte in vitro. Methods:A model of non-alcoholic fatty liver disease(NAFLD)oleic acid-induced steatosis L-O2 strain hepatocyte(normal adult hepatocytes)was established. The model was divided into normal group

steatosis model group

prevention group and treatment group. The accumulation of lipid droplets in hepatocytes was observed under light microscopy after oil red-O staining after 48 h. The levels of alanine aminostransferase(ALT) aspartate aminostransferase(AST) triglyceride(TG) and the expression of m RNA and protein of adiponectin and resistin were detected.Results:Much more obvious orange lipid droplets in hepatocytes were found in model group after culture than in normal group(P=0.000). Lipid droplets decreased in prevention group and treatment group than in model group(P=0.000;P=0.000). The levels of ALT

AST and TG were decreased(P=0.000;P=0.000;P=0.000);the expressions of m RNA and protein of adiponectin were up-regulated(P=0.000;P=0.014)and the expressions of m RNA and protein of resistin were down-regulated(P=0.000;P=0.000). Conclusion:Induced by expression of m RNA and protein of adiponectin and resistin

NAFLD can be prevented and treated effectively by LBP.