经内镜逆行胰胆管造影术后胰腺炎患者外周血TFEB、TREM-1表达与病情的关系

陈昭伟; 康丽丽; 宋健; 赵心恺

doi:10.11659/jjssx.12E022080

您当前的位置：

首页 >

文章列表页 >

经内镜逆行胰胆管造影术后胰腺炎患者外周血TFEB、TREM-1表达与病情的关系

临床研究 | 更新时间：2023-07-31

- 经内镜逆行胰胆管造影术后胰腺炎患者外周血TFEB、TREM-1表达与病情的关系
- Relationship between the expression of TFEB and TREM-1 in peripheral blood of patients with pancreatitis after endoscopic retrograde cholangiopancreatography and their condition
- 局解手术学杂志 2023年32卷第8期页码：692-695
- 作者机构：
  
  海南省肿瘤医院消化内镜科，海南海口 570100
- 作者简介：
  
  赵心恺，E-mail：13700477665@163.com
- 基金信息：
  
  2020年海南省重大科技计划项目(ZDKJ202005)
- DOI：10.11659/jjssx.12E022080
  中图分类号： R576
- 纸质出版日期：2023-08-25，
  
  收稿日期：2022-12-15，
扫描看全文
陈昭伟,康丽丽,宋健等.经内镜逆行胰胆管造影术后胰腺炎患者外周血TFEB、TREM-1表达与病情的关系[J].局解手术学杂志,2023,32(08):692-695.

CHEN Zhao-wei,KANG Li-li,SONG Jian,et al.Relationship between the expression of TFEB and TREM-1 in peripheral blood of patients with pancreatitis after endoscopic retrograde cholangiopancreatography and their condition[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(08):692-695.
陈昭伟,康丽丽,宋健等.经内镜逆行胰胆管造影术后胰腺炎患者外周血TFEB、TREM-1表达与病情的关系[J].局解手术学杂志,2023,32(08):692-695. DOI： 10.11659/jjssx.12E022080.

CHEN Zhao-wei,KANG Li-li,SONG Jian,et al.Relationship between the expression of TFEB and TREM-1 in peripheral blood of patients with pancreatitis after endoscopic retrograde cholangiopancreatography and their condition[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(08):692-695. DOI： 10.11659/jjssx.12E022080.

摘要

目的

探讨行内镜逆行胰胆管造影术（ERCP）后胰腺炎（PEP）患者外周血单个核细胞中转录因子EB（TFEB）、髓样细胞触发受体-1（TREM-1）表达与病情程度的关系。

方法

选取2019年6月至2022年3月在本院就诊的PEP患者105例为观察对象，设为PEP组，根据患者病情严重程度将其分为轻度组（62例）、中度组（32例）和重度组（11例）；选择同期ERCP后未发生PEP患者110例作为对照组。实时荧光定量PCR法检测患者手术前后外周血单个核细胞中TFEB、TREM-1表达水平；采用免疫比浊法检测手术前后血清C反应蛋白（CRP）水平；采用ELISA法检测血清白细胞介素-1（IL-1）、白细胞介素-6（IL-6）水平。术后对患者进行急性生理健康与慢性疾病（APACHE Ⅱ）评分；

Spearman

法分析PEP患者外周血TFEB、TREM-1表达水平与APACHE Ⅱ评分的相关性；

Pearson

法分析PEP患者外周血TFEB、TREM-1表达水平与CRP、IL-1、IL-6的相关性。

结果

PEP组术后血清CRP、IL-1、IL-6水平显著高于术前，且PEP组高于对照组（

0.05）。与术前比较，PEP组术后外周血TFEB表达水平显著降低，TREM-1表达水平显著升高（

0.05）；与对照组比较，PEP组术后外周血TFEB表达水平显著降低，TREM-1表达水平显著升高（

0.05）。与轻度组比较，中度组、重度组PEP患者术后外周血TFEB表达水平显著降低，TREM-1表达水平及APACHEⅡ评分显著升高（

0.05）；与中度组比较，重度组PEP患者术后外周血TFEB表达水平显著降低，TREM-1表达水平及APACHEⅡ评分显著升高（

0.05）。相关性分析结果显示，外周血TFEB表达水平与APACHEⅡ评分、血清CRP、IL-1、IL-6水平呈负相关（

0.05），外周血TREM-1表达水平与APACHEⅡ评分、血清CRP、IL-1、IL-6水平呈正相关（

0.05）。

结论

PEP患者外周血单个核细胞中TFEB表达水平降低，TREM-1表达水平升高，TFEB、TREM-1与患者病情严重程度均相关，可用于评估PEP病情。

Abstract

Objective

To investigate the relationship between the expression of transcription factor EB （TFEB） and triggering receptor expressed on myeloid cells-1 （TREM-1） in peripheral blood mononuclear cells of patients with pancreatitis （PEP） after endoscopic retrograde cholangiopancreatography （ERCP） and the severity of the disease.

Methods

A total of 105 PEP patients who were treated in our hospital from June 2019 to March 2022 were selected as the observation objects and set as the PEP group. According to the severity of the patients' conditions， they were divided into the mild group （62 cases）， the moderate group （32 cases） and the severe group （11 cases）. A total of 110 patients without PEP after ERCP during the same period were selected as the control group. The expression levels of TFEB and TREM-1 in peripheral blood mononuclear cells before and after surgery were detected by Real-time fluorescence quantitative PCR. The

levels of serum C-reactive protein （CRP） before and after surgery were detected by immunoturbidimetry. The levels of serum interleukin-1 （IL-1））and interleukin-6 （IL-6） were detected by ELISA. The acute physiology and chronic health evaluation Ⅱ （APACHE Ⅱ） score was performed on the patients after operation.

Spearman

method was used to analyze the correlation between the expression levels of TFEB and TREM-1 in peripheral blood of PEP patients and the APACHE Ⅱ score.

Pearson

method was used to analyze the correlation between the expression levels of TFEB and TREM-1 in peripheral blood of PEP patients and CRP， IL-1 and IL-6.

Results

The levels of serum CRP， IL-1 and IL-6 after operation in the PEP group were significantly higher than those before operation， and those in the PEP group were higher than those in the control group （

0.05）. Compared with before operation， the expression level of TFEB in peripheral blood after operation in the PEP group was significantly decreased， and the expression level of TREM-1 was significantly increased （

0.05）. Compared with the control group， the expression level of TFEB in peripheral blood after operation in the PEP group was significantly decreased， while the expression level of TREM-1 was significantly increased （

0.05）. Compared with the mild group， the expression levels of TFEB in peripheral blood of PEP patients after operation in the moderate group and the severe group were significantly decreased， while the expression levels of TREM-1 and APACHE Ⅱ score were significantly increased （

0.05）. Compared with the moderate group， the expression level of TFEB in peripheral blood of PEP patients after operation in the severe group was significantly decreased， while the expression level of TREM-1 and APACHE Ⅱ score were significantly increased （

0.05）. The results of correlation analysis showed that the expression level of TFEB in peripheral blood was negatively correlated with APACHE Ⅱ score， serum CRP， IL-1 and IL-6 levels （

0.05）， while the expression level of TREM-1 in peripheral blood was positively correlated with APACHE Ⅱ score， serum CRP， IL-1 and IL-6 levels （

0.05）.

Conclusion

The expression level of TFEB in peripheral blood mononuclear cells of PEP patients is decreased， while the expression level of TREM-1 is increased. TFEB and TREM-1 are related to the severity of the patients' disease， which can be used to evaluate the condition of PEP.

关键词

内镜逆行胰胆管造影术胰腺炎转录因子EB髓样细胞触发受体-1外周血单个核细胞

Keywords

endoscopic retrograde cholangiopancreatographypancreatitistranscription factor EBtriggering receptor expressed on myeloid cells-1peripheral blood mononuclear cells

references

Suzuki A，Uno K，Nakase K，et al.Post-endoscopic retrograde cholangiopancreatography pancreatitis assessed using criteria for acute pancreatitis［J］.JGH Open，2021，5（12）：1391-1397.doi：10.1002/jgh3.12687http://dx.doi.org/10.1002/jgh3.12687.

李婧伊，刘飞，马跃峰，等.年龄对ERCP治疗胆总管结石术后并发胰腺炎及严重程度的影响［J］.中国普通外科杂志，2020，28（8）：936-942.doi：10.7659/j.issn.1005-6947.2019.08.005http://dx.doi.org/10.7659/j.issn.1005-6947.2019.08.005.

Li R，Zhao X，Zhang S，et al.RIP3 impedes transcription factor EB to suppress autophagic degradation in septic acute kidney injury［J］.Cell Death Dis，2021，12（6）：593-609.doi：10.1038/s41419-021-03865-8http://dx.doi.org/10.1038/s41419-021-03865-8.

Wang S，Ni HM，Chao X，et al.Impaired TFEB-mediated lysosomal biogenesis promotes the development of pancreatitis in mice and is associated with human pancreatitis［J］.Autophagy，2019，15（11）：1954-1969.doi：10.1080/15548627.2019.1596486http://dx.doi.org/10.1080/15548627.2019.1596486.

Pan P，Liu X，Wu L，et al.TREM-1 promoted apoptosis and inhibited autophagy in LPS-treated HK-2 cells through the NF-κB pathway［J］.Int J Med Sci，2021，18（1）：8-17.doi：10.7150/ijms.50893http://dx.doi.org/10.7150/ijms.50893.

云宇婷，时牛，宋洁，等.AP患者外周血TREM-1、MIP-1α、proBNP的表达水平及其与病情严重程度的关系［J］.川北医学院学报，2022，37（1）：31-34.doi：10.3969/j.issn.1005-3697. 2022.01.007http://dx.doi.org/10.3969/j.issn.1005-3697.2022.01.007.

Banks PA，Bollen TL，Dervenis C，et al.Classification of acute pancreatitis--2012：revision of the Atlanta classification and definitions by international consensus［J］.Gut，2013，62（1）：102-111.doi：10.1136/gutjnl-2012-302779http://dx.doi.org/10.1136/gutjnl-2012-302779.

Liu X，Zheng X，Lu Y，et al.TFEB dependent autophagy-lysosomal pathway：an emerging pharmacological target in sepsis［J］.Front Pharmacol，2021，12（1）：1-9.doi：10.3389/fphar.2021.794298http://dx.doi.org/10.3389/fphar.2021.794298.

Wang S，Ni HM，Chao X，et al.Critical Role of TFEB-mediated lysosomal biogenesis in alcohol-induced pancreatitis in mice and humans［J］.Cell Mol Gastroenterol Hepatol，2020，10（1）：59-81.doi：10.1016/j.jcmgh.2020.01.008http://dx.doi.org/10.1016/j.jcmgh.2020.01.008.

Li B，Wu J，Bao J，et al.Activation of α7nACh receptor protects against acute pancreatitis through enhancing TFEB-regulated autophagy［J］.Biochim Biophys Acta Mol Basis Dis，2020，1866（12）：1-11.doi：10.1016/j.bbadis.2020.165971http://dx.doi.org/10.1016/j.bbadis.2020.165971.

Wang X，Wang Q，Li W，et al.TFEB-NF-κB inflammatory signaling axis：a novel therapeutic pathway of Dihydrotanshinone I in doxorubicin-induced cardiotoxicity［J］.J Exp Clin Cancer Res，2020，39（1）：93-108.doi：10.1186/s13046-020-01595-xhttp://dx.doi.org/10.1186/s13046-020-01595-x.

Liu W，Li CC，Lu X，et al.Overexpression of transcription factor EB regulates mitochondrial autophagy to protect lipopolysaccharide-induced acute lung injury［J］.Chin Med J （Engl），2019，132（11）：1298-1304.doi：10.1097/CM9.0000000000000243http://dx.doi.org/10.1097/CM9.0000000000000243.

何昱静，温雪，卢利霞，等.自噬、炎症反应及免疫功能异常在急性胰腺炎中的研究进展［J］.胃肠病学和肝病学杂志，2022，31（1）：104-109.doi：10.3969/j.issn.1006-5709.2022.01.021http://dx.doi.org/10.3969/j.issn.1006-5709.2022.01.021.

Rao S，Huang J，Shen Z，et al.Inhibition of TREM-1 attenuates inflammation and lipid accumulation in diet-induced nonalcoholic fatty liver disease［J］.J Cell Biochem，2019，120（7）：11867-11877.doi：10.1002/jcb.28468http://dx.doi.org/10.1002/jcb.28468.

吴雅娟，刘蓉，董凌云，等.胸腔积液病人体内TREM-1和Treg细胞、DNT细胞的变化特征及其与疾病鉴别的关系［J］.免疫学杂志，2021，37（6）：535-540.doi：10.13431/j.cnki.immunol.j. 20210073http://dx.doi.org/10.13431/j.cnki.immunol.j.20210073.

陈怿，邱嘉玲，丁程佳，等.单核-巨噬细胞TREM-1对重症中暑大鼠急性肺损伤的作用及机制［J］.解放军医学杂志，2020，45（1）：62-67.doi：10.11855/j.issn.0577-7402.2020.01.06http://dx.doi.org/10.11855/j.issn.0577-7402.2020.01.06.

Wang L，Chen Q，Yu Q，et al.TREM-1 aggravates chronic obstructive pulmonary disease development via activation NLRP3 inflammasome-mediated pyroptosis［J］.Inflamm Res，2021，70（9）：971-980.doi：10.1007/s00011-021-01490-xhttp://dx.doi.org/10.1007/s00011-021-01490-x.

董玉，闫凯，程海玲.TREM-1、H2S联合ACE2检测对重症急性胰腺炎预后的评估价值［J］.分子诊断与治疗杂志，2021，13（2）：284-287.doi：10.3969/j.issn.1674-6929.2021.02.029http://dx.doi.org/10.3969/j.issn.1674-6929.2021.02.029.

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

1 800 MHz电磁辐射暴露对大鼠外周血单个核细胞代谢组的影响

经皮穿刺置管引流术在急性胰腺炎合并坏死感染中的治疗效果

吲哚美辛栓预防十二指肠乳头小切开联合球囊扩张取石术后胰腺炎的作用