浏览全部资源
扫码关注微信
circDCUN1D4调节miR-18a-5p/FBP1轴对肺癌细胞增殖、凋亡和免疫逃逸的影响
Effect of circDCUN1D4 on proliferation,apoptosis and immune escape of lung cancer cells by regulating the miR-18a-5p/FBP1 axis
- 局解手术学杂志 2024年33卷第1期 页码：19-23
- 作者机构：
1.宝鸡市中医医院肿瘤科，陕西 宝鸡 721000
2.宝鸡市中医医院呼吸与危重症医学科，陕西 宝鸡 721000
3.西安国际医学中心医院呼吸内科，陕西 西安 710000
- 作者简介：
蔺建平，E-mail：iqqnu61@163.com
- 基金信息：陕西省自然科学基础研究计划项目(S2021-JC-QN-2452)
DOI：10.11659/jjssx.12E022012
中图分类号： R734.2纸质出版日期：2024-01-25，
收稿日期：2022-12-02，
扫 描 看 全 文
史春辉,蔺建平,吴超.circDCUN1D4调节miR-18a-5p/FBP1轴对肺癌细胞增殖、凋亡和免疫逃逸的影响[J].局解手术学杂志,2024,33(01):19-23.
SHI Chun-hui,LIN Jian-ping,WU Chao.Effect of circDCUN1D4 on proliferation,apoptosis and immune escape of lung cancer cells by regulating the miR-18a-5p/FBP1 axis[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2024,33(01):19-23.
史春辉,蔺建平,吴超.circDCUN1D4调节miR-18a-5p/FBP1轴对肺癌细胞增殖、凋亡和免疫逃逸的影响[J].局解手术学杂志,2024,33(01):19-23. DOI： 10.11659/jjssx.12E022012.
SHI Chun-hui,LIN Jian-ping,WU Chao.Effect of circDCUN1D4 on proliferation,apoptosis and immune escape of lung cancer cells by regulating the miR-18a-5p/FBP1 axis[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2024,33(01):19-23. DOI： 10.11659/jjssx.12E022012.
摘要
目的
2
探讨环状RNA（circRNA）DCUN1D4调节微小RNA（miR）-18a-5p/果糖-1
6-二磷酸酶1（FBP1）轴对肺癌细胞增殖、凋亡和免疫逃逸的影响。
方法
2
选取人肺癌细胞系（H1975、H1650、A549、SPCA-1）和人正常肺表皮细胞（HPL-1），qRT-PCR检测各种细胞中circDCUN1D4、miR-18a-5p、FBP1 mRNA表达水平。取对数生长期的A549细胞并分为空白组、circDCUN1D4过表达质粒（circDCUN1D4）组、过表达质粒阴性对照（NC）组、circDCUN1D4+miR-18a-5p模拟物阴性对照（circDCUN1D4+mimics NC）组、circDCUN1D4+miR-18a-5p模拟物（circDCUN1D4+miR-18a-5p mimics）组。CCK-8法检测各组A549细胞活力，流式细胞术检测各组A549细胞凋亡水平，qRT-PCR检测各组A549细胞中circDCUN1D4、miR-18a-5p、FBP1 mRNA表达水平，Western blot检测各组A549细胞中FBP1、caspase-3、Ki67、增殖细胞核抗原（PCNA）及程序性死亡分子配体-1（PD-L1）表达水平，双荧光素酶实验验证miR-18a-5p分别与circDCUN1D4、FBP1的靶向关系。
结果
2
与HPL-1细胞相比，人肺癌细胞系中circDCUN1D4、FBP1 mRNA表达显著下降（
P
<
0.05），miR-18a-5p表达显著增加（
P
<
0.05）。miR-18a-5p分别与circDCUN1D4、FBP1存在靶向关系。与空白组、NC组相比，circDCUN1D4组细胞24 h和48 h的OD值、Ki67、PCNA、miR-18a-5p、PD-L1表达显著降低（
P
<
0.05），细胞凋亡率、circDCUN1D4、FBP1、caspase-3表达显著增加（
P
<
0.05）；过表达miR-18a-5p逆转了circDCUN1D4对肺癌细胞恶性行为的抑制作用（
P
<
0.05）。
结论
2
circDCUN1D4过表达可以促进肺癌细胞凋亡，抑制肺癌细胞增殖及免疫逃逸，其作用机制可能与调控miR-18a-5p/FBP1轴有关。
Abstract
Objective
2
To investigate the influences of circular RNA （circRNA） DCUN1D4 on the proliferation，apoptosis and immune escape of lung cancer cells by regulating the microRNA （miR）-18a-5p/fructose-1，6-bisphosphatase 1 （FBP1） axis.
Methods
2
The human lung cancer cell lines H1975，H1650，A549 and SPCA-1 and human normal lung epidermal cells HPL-1 were selected，qRT-PCR was used to detect the expression levels of circDCUN1D4，miR-18a-5p and FBP1 mRNA in various cells.A549 cells in logarithmic growth phase were selected and divided into the blank group，circDCUN1D4 overexpression plasmid （circDCUN1D4） group，overexpression plasmid negative control （NC） group，circDCUN1D4+miR-18a-5p mimics negative control （circDCUN1D4+mimics NC） group，and circDCUN1D4+miR-18a-5p mimics group. The cell viability of each group was detected by CCK-8 method，the cell apoptosis was detected by flow cytometry，the expression levels of circDCUN1D4，miR-18a-5p and FBP1 mRNA of cells in each group were detected by qRT-PCR，the expression levels of FBP1，caspase-3，Ki67，proliferating cell nuclear antigen （PCNA） and programmed death ligand-1 （PD-L1）were detected by Western blot，the targeting relationships of miR-18a-5p with circDCUN1D4 and FBP1 were verified by dual luciferase assay.
Results
2
Compared with HPL-1 cells，the mRNA expressions of circDCUN1D4 and FBP1 were significantly decreased （
P
<
0.05），and the expression of miR-18a-5p was significantly increased （
P
<
0.05）.miR-18a-5p had targeting relationships with circDCUN1D4 and FBP1，respectively. Compared with the blank group and NC group，the OD values at 24 hours and 48 hours，and the expressions of Ki67，PCNA，miR-18a-5p and PD-L1 of cells in the circDCUN1D4 group were significantly decreased（
P
<
0.05），and the apoptosis rate，and the expressions of circDCUN1D4，FBP1 and caspase-3 were significantly increased （
P
<
0.05）.Overexpression of miR-18a-5p reversed the inhibitory effect of circDCUN1D4 on the malignant behavior of lung cancer cells （
P
<
0.05）.
Conclusion
2
Overexpression of circDCUN1D4 can promote lung cancer cell apoptosis，inhibit lung cancer cell proliferation and immune escape，and its mechanism may be related to the regulation of miR-18a-5p/FBP1 axis.
关键词
circDCUN1D4miR-18a-5p/FBP1轴肺癌增殖凋亡免疫逃逸
Keywords
circDCUN1D4miR-18a-5p/FBP1 axislung cancerproliferationapoptosisimmune escape
references
邵为朋，王晓伟，刘德若.小细胞肺癌目前治疗的策略与未来方向［J］.中国肺癌杂志，2017，20（6）：421-426.doi：10.3779/j.issn.1009-3419.2017.06.09http://dx.doi.org/10.3779/j.issn.1009-3419.2017.06.09.
陈天佑，熊飞，张倬，等.LncRNA IGF2-AS基因通过靶向miR-370-3p对肺癌细胞增殖和凋亡的影响［J］.中国老年学杂志，2021，41（23）：5308-5313.doi：10.3969/j.issn.1005-9202http://dx.doi.org/10.3969/j.issn.1005-9202.
国家癌症中心，国家肿瘤质控中心肺癌质控专家委员会.中国原发性肺癌规范诊疗质量控制指标（2022版）［J］.中华肿瘤杂志，2022，44（12）：1235-1241.doi：10.3760/cma.j.cn112152-20220418-00266http://dx.doi.org/10.3760/cma.j.cn112152-20220418-00266.
Kristensen LS，Andersen MS，Stagsted LVW，et al.The biogenesis，biology and characterization of circular RNAs［J］.Nat Rev Genet，2019，20（11）：675-691.doi：10.1038/s41576-019-0158-7http://dx.doi.org/10.1038/s41576-019-0158-7.
Li B，Zhu L，Lu C，et al.circNDUFB2 inhibits non-small cell lung cancer progression via destabilizing IGF2BPs and activating anti-tumor immunity［J］.Nat Commun，2021，12（1）：295.doi：10.1038/s41467-020-20527-zhttp://dx.doi.org/10.1038/s41467-020-20527-z.
Vo JN，Cieslik M，Zhang Y，et al.The landscape of circular RNA in cancer［J］.Cell，2019，176（4）：869-881.e13.doi：10.1016/j.cell.2018.12.021http://dx.doi.org/10.1016/j.cell.2018.12.021.
Friedman RC，Farh KK，Burge CB，et al.Most mammalian mRNAs are conserved targets of microRNAs［J］.Genome Res，2009，19（1）：92-105.doi：10.1101/gr.082701.108http://dx.doi.org/10.1101/gr.082701.108.
Quiñones-Díaz BI，Reyes-González JM，Sánchez-Guzmán V，et al.MicroRNA-18a-5p suppresses tumor growth via targeting matrix metalloproteinase-3 in cisplatin-resistant ovarian cancer［J］.Front Oncol，2020，10：602670.doi：10.3389/fonc.2020.602670http://dx.doi.org/10.3389/fonc.2020.602670.
Bray F，Ferlay J，Soerjomataram I，et al.Global cancer statistics 2018：GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries［J］.CA Cancer J Clin，2018，68（6）：394-424.doi：10.3322/caac.21492http://dx.doi.org/10.3322/caac.21492.
Bade BC，Dela Cruz CS.Lung cancer 2020：epidemiology，etiology，and prevention［J］.Clin Chest Med，2020，41（1）：1-24.doi：10.1016/j.ccm.2019.10.001http://dx.doi.org/10.1016/j.ccm.2019.10.001.
Li MY，Liu LZ，Dong M.Progress on pivotal role and application of exosome in lung cancer carcinogenesis，diagnosis，therapy and prognosis［J］.Mol Cancer，2021，20（1）：22.doi：10.1186/s12943-021-01312-yhttp://dx.doi.org/10.1186/s12943-021-01312-y.
Chen L，Nan A，Zhang N，et al.Circular RNA 100146 functions as an oncogene through direct binding to miR-361-3p and miR-615-5p in non-small cell lung cancer［J］.Mol Cancer，2019，18（1）：13.doi：10.1186/s12943-019-0943-0http://dx.doi.org/10.1186/s12943-019-0943-0.
Li P，Chen H，Chen S，et al.Circular RNA 0000096 affects cell growth and migration in gastric cancer［J］.Br J Cancer，2017，116（5）：626-633.doi：10.1038/bjc.2016.451http://dx.doi.org/10.1038/bjc.2016.451.
Wang J，Zhao X，Wang Y，et al.circRNA-002178 act as a ceRNA to promote PDL1/PD1 expression in lung adenocarcinoma［J］.Cell Death Dis，2020，11（1）：32.doi：10.1038/s41419-020-2230-9http://dx.doi.org/10.1038/s41419-020-2230-9.
Liang Y，Wang H，Chen B，et al.circDCUN1D4 suppresses tumor metastasis and glycolysis in lung adenocarcinoma by stabilizing TXNIP expression［J］.Mol Ther Nucleic Acids，2020，23：355-368.doi：10.1016/j.omtn.2020.11.012http://dx.doi.org/10.1016/j.omtn.2020.11.012.
冯涛，肖向茜，盛望.miRNA在肺癌及肺癌耐药中的作用机制及治疗的研究进展［J］.医学综述，2018，24（1）：61-65.doi：10.3969/j.issn.1006-2084.2018.01.012http://dx.doi.org/10.3969/j.issn.1006-2084.2018.01.012.
Cui M，Qu F，Wang L，et al.MiR-18a-5p facilitates progression of hepatocellular carcinoma by targeting CPEB3［J］.Technol Cancer Res Treat，2021，20：15330338211043976.doi：10.1177/15330 338211043976http://dx.doi.org/10.1177/15330338211043976.
Guo Y，Shi W，Fang R.miR-18a-5p promotes melanoma cell proliferation and inhibits apoptosis and autophagy by targeting EPHA7 signaling［J］.Mol Med Rep，2021，23（1）：79.doi：10.3892/mmr.2020.11717http://dx.doi.org/10.3892/mmr.2020.11717.
Gao S，Zhu D，Zhu J，et al.miR-18a-5p targets FBP1 to promote proliferation，migration，and invasion of liver cancer cells and inhibit cell apoptosis［J］.Comput Math Methods Med，2021，2021：3334065.doi：10.1155/2021/3334065http://dx.doi.org/10.1155/2021/3334065.
Zhang J，Wang J，Xing H，et al.Down-regulation of FBP1 by ZEB1-mediated repression confers to growth and invasion in lung cancer cells［J］.Mol Cell Biochem，2016，411（1/2）：331-340.doi：10.1007/s11010-015-2595-8http://dx.doi.org/10.1007/s11010-015-2595-8.
Zhang Y，Lv C，Dong Y，et al.Aspirin-targeted PD-L1 in lung cancer growth inhibition［J］.Thorac Cancer，2020，11（6）：1587-1593.doi：10.1111/1759-7714.13433http://dx.doi.org/10.1111/1759-7714.13433.
0
浏览量
2
下载量
0
CSCD
- 网络PDF下载
- WORD下载
- Meta-XML下载
- BibTeX下载
- Endnote下载
- RefMan 下载
- NoteExpress下载
- NoteFirst下载
关联资源
相关文章
相关作者
相关机构
- 技术支持由北京北大方正电子有限公司提供 京ICP备09064830号-19
京公网安备11010802024621 - 本系统建议在Chrome、 IE9+ 以上版本浏览器阅读本站内容，360浏览器请切换至极速模式
- Cookies帮助我们提供服务并提供个性化体验。使用本网站，即表示您同意我们使用Cookies
