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乳腺癌miR-4500、NGF表达与术前磁共振征象的相关性研究
Correlation analysis of miR-4500, NGF expressions with preoperative magnetic resonance signs in breast cancer
- 局解手术学杂志 2024年33卷第1期 页码：47-50
- 作者机构：
河北北方学院附属第一医院医学影像部，河北 张家口 075000
- 作者简介：
杨帆，E-mail：yangfan1993yangfan@163.com
- 基金信息：河北省2021年度医学科学研究课题计划(20210136)
DOI：10.11659/jjssx.12E022002
中图分类号： R445.2;R737.9纸质出版日期：2024-01-25，
收稿日期：2022-12-01，
扫 描 看 全 文
杨帆,陈静,张斌等.乳腺癌miR-4500、NGF表达与术前磁共振征象的相关性研究[J].局解手术学杂志,2024,33(01):47-50.
YANG Fan,CHEN Jing,ZHENG Bin,et al.Correlation analysis of miR-4500, NGF expressions with preoperative magnetic resonance signs in breast cancer[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2024,33(01):47-50.
杨帆,陈静,张斌等.乳腺癌miR-4500、NGF表达与术前磁共振征象的相关性研究[J].局解手术学杂志,2024,33(01):47-50. DOI： 10.11659/jjssx.12E022002.
YANG Fan,CHEN Jing,ZHENG Bin,et al.Correlation analysis of miR-4500, NGF expressions with preoperative magnetic resonance signs in breast cancer[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2024,33(01):47-50. DOI： 10.11659/jjssx.12E022002.
摘要
目的
2
分析乳腺癌患者中miR-4500、神经生长因子（NGF）表达与术前磁共振（MR）征象的相关性。
方法
2
选取我院收治的乳腺癌患者105例，通过qRT-PCR法检测患者乳腺癌组织及癌旁组织中miR-4500、NGF mRNA表达水平，免疫组化法测定乳腺癌组织及癌旁组织中NGF的阳性表达率。分析乳腺癌组织中miR-4500、NGF表达与患者临床病理特征及MR征象的相关性。生物信息网站预测miR-4500与NGF的靶向关系，并分析二者表达的相关性。
结果
2
与癌旁组织相比，乳腺癌组织中miR-4500表达水平降低（
P
<
0.05），NGF mRNA水平、NGF阳性表达率升高（
P
<
0.05）。年龄、病理类型、肿瘤分型、实质背景、表观弥散系数、强化曲线在不同miR-4500表达及NGF表达间差异无统计学意义（
P
>
0.05），组织学分级、淋巴结转移、肿瘤直径、肿瘤形态、环形强化、瘤周水肿与miR-4500表达及NGF表达有关（
P
<
0.05）。生物信息网站预测显示，miR-4500与NGF存在结合位点，乳腺癌组织中miR-4500表达与NGF mRNA表达呈负相关（
r
=-0.576，
P
<
0.05）。
结论
2
乳腺癌组织miR-4500低表达，NGF高表达，二者与患者术前MR征象具有相关性，为MR征象提供了分子学依据。
Abstract
Objective
2
To analyze the correlations of the expressions of miR-4500 and nerve growth factor （NGF） in breast cancer patients with preoperative magnetic resonance （MR） signs.
Methods
2
A total of 105 patients with breast cancer admitted to our hospital were selected， the mRNA expression levels of miR-4500 and NGF in breast cancer tissues and adjacent tissues of patients were detected by qRT-PCR， and the positive expression rates of NGF in breast cancer tissues and adjacent tissues were determined by immunohistochemistry method. The correlations of the expressions of miR-4500 and NGF in breast cancer tissues with clinicopathological features and MR signs of patients were analyzed. The targeting relationship between miR-4500 and NGF was predicted by the bioinformatics website， and the correlation between the expressions of the two was analyzed.
Results
2
Compared with the adjacent tissues， the expression level of miR-4500 in breast cancer tissue decreased （
P
<
0.05）， while the level of NGF mRNA and the positive expression rate of NGF increased （
P
<
0.05）.There was no significant difference in age， pathological type， tumor classification， parenchymal background， apparent diffusion coefficient or enhancement curve among different expressions of miR-4500 and NGF （
P
>
0.05）. The histological grade， lymph node metastasis， tumor diameter， tumor morphology， ring-like enhancement， and peritu-moral brain edema were related to the expressions of miR-4500 and NGF（
P
<
0.05）. The bioinformatics website prediction showed that miR-4500 and NGF had binding sites， and the expressions of miR-4500 and NGF mRNA in breast cancer tissues were negatively correlated （
r
=-0.576，
P
<
0.05）.
Conclusion
2
The expression of miR-4500 in breast cancer tissue is low， and the expression of NGF is high， which are correlated with the preoperative MR signs in patients， providing a molecular basis for MR signs.
关键词
乳腺癌miR-4500神经生长因子MR征象
Keywords
breast cancermiR-4500nerve growth factorMR signs
references
da Silva BAO，Dias IS，Sarto LE，et al.Cytotoxicity induced by newly synthesized palladium （Ⅱ） complexes lead to the death of MCF-7 and MDA-MB-435 cancer cell lines［J］.Adv Pharm Bull，2023，13（1）：160-169.doi：10.34172/apb.2023.017http://dx.doi.org/10.34172/apb.2023.017.
Min J，Kim JY，Yeon S，et al.Change in shoulder function in the early recovery phase after breast cancer surgery：a prospective observational study［J］.J Clin Med，2021，10（15）：3416.doi：10.3390/jcm10153416http://dx.doi.org/10.3390/jcm10153416.
Khalki LE，Maire V，Dubois T，et al.Berberine impairs the survival of triple negative breast cancer cells：cellular and molecular analyses［J］.Molecules，2020，25（3）：506.doi：10.3390/molecules25030506http://dx.doi.org/10.3390/molecules25030506.
呙月，刘诗月，黄玉琴，等.miR-4500通过转录后下调LIN28B表达抑制乳腺癌细胞的迁移和增殖［J］.山西医科大学学报，2020，51（2）：119-123.doi：10.13753/j.issn.1007-6611.2020.02.003http://dx.doi.org/10.13753/j.issn.1007-6611.2020.02.003.
Li S，Mai H，Zhu Y，et al.MicroRNA-4500 inhibits migration，invasion and angiogenesis of breast cancer cells via RRM2-dependent MAPK signaling pathway［J］.Mol Ther Nucleic Acids，2020，21：278-289.doi：10.1016/j.omtn.2020.04.018http://dx.doi.org/10.1016/j.omtn.2020.04.018.
Jafari M，Delaviz H，Torabi S，et al.The effect of muscle graft with nerve growth factor and laminin on sciatic nerve repair in rats［J］.Basic Clin Neurosci，2019，10（4）：333-344.doi：10.32598/bcn.9.10.145http://dx.doi.org/10.32598/bcn.9.10.145.
Bruno F，Arcuri D，Vozzo F，et al.Expression and signaling pathways of nerve growth factor （NGF） and pro-NGF in breast cancer：a systematic review［J］.Curr Oncol，2022，29（11）：8103-8120.doi：10.3390/curroncol29110640http://dx.doi.org/10.3390/curroncol29110640.
朱景航，高小玲.MR征象与乳腺癌VEGF表达的相关性分析［J］.影像诊断与介入放射学，2021，30（4）：261-265.doi：10.3969/j.issn.1005-8001.2021.04.004http://dx.doi.org/10.3969/j.issn.1005-8001.2021.04.004.
Wilkinson L，Gathani T.Understanding breast cancer as a global health concern［J］.Br J Radiol，2022，95（1130）：20211033.doi：10.1259/bjr.20211033http://dx.doi.org/10.1259/bjr.20211033.
Ding W，Fan Z，Xu Y，et al.Magnetic resonance imaging in screening women at high risk of breast cancer：a meta-analysis［J］.Medicine （Baltimore），2023，102（10）：e33146.doi：10.1097/MD.0000000000033146http://dx.doi.org/10.1097/MD.0000000000033146.
Bruno F，Abondio P，Montesanto A，et al.The nerve growth factor receptor （NGFR/p75NTR）：a major player in Alzheimer's disease［J］.Int J Mol Sci，2023，24（4）：3200.doi：10.3390/ijms24043200http://dx.doi.org/10.3390/ijms24043200.
Vera DB，Fredes AN，Garrido MP，et al.Role of mitochondria in interplay between NGF/TRKA，miR-145 and possible therapeutic strategies for epithelial ovarian cancer［J］.Life，2021，12（1）：8.doi：10.3390/life12010008http://dx.doi.org/10.3390/life12010008.
Mazaki A，Yamauchi K，Orita S，et al.Nerve growth factor in breast cancer cells promotes axonal growth and expression of calcitonin gene-related peptide in a rat model of spinal metastasis［J］.Anticancer Res，2022，42（1）：581-587.doi：10.21873/anticanres.15515http://dx.doi.org/10.21873/anticanres.15515.
Li ZY，Zhang ZZ，Bi H，et al.MicroRNA4500 suppresses tumor progression in nonsmall cell lung cancer by regulating STAT3［J］.Mol Med Rep，2019，20（6）：4973-4983.doi：10.3892/mmr.2019.10737http://dx.doi.org/10.3892/mmr.2019.10737.
Fu D，Ren Y，Wang C，et al.LINC01287 facilitates proliferation，migration，invasion and EMT of colon cancer cells via miR-4500/MAP3K13 pathway［J］.BMC Cancer，2021，21（1）：782.doi：10.1186/s12885-021-08528-7http://dx.doi.org/10.1186/s12885-021-08528-7.
Peng W，Dong N，Wu S，et al.miR‐4500 suppresses cell proliferation and migration in bladder cancer via inhibition of STAT3/CCR7 pathway［J］.J Cell Biochem，2019，121（8/9）：3913-3922.doi：10.1002/jcb.29558http://dx.doi.org/10.1002/jcb.29558.
岳叶洼团，熊春来.磁共振T2WI序列瘤周水肿征象用于润性乳腺癌早期复发诊断的价值分析［J］.影像研究与医学应用，2021，5（21）：44-46.doi：10.3969/j.issn.2096-3807.2021.21.021http://dx.doi.org/10.3969/j.issn.2096-3807.2021.21.021.
Lee HJ，Lee JE，Jeong WG，et al.HER2-positive breast cancer：association of MRI and clinic opathologic features with tumor-infiltrating lymphocytes［J］.AJR Am J Roentgenol，2022，218（2）：258-269.doi：10.2214/AJR.21.26400http://dx.doi.org/10.2214/AJR.21.26400.
黎冠峰.MR动态增强对不同组织类型乳腺癌的诊断及预后评价［J］.现代医用影像学，2019，28（2）：240-243.
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