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lncRNA NEAT1通过抑制miR-let-7a激活BZW2促进喉乳头状瘤细胞的增殖和凋亡逃逸
lncRNA NEAT1 promoting the proliferation and apoptosis escape of laryngeal papilloma cells by activating BZW2 through the inhibition of miR-let-7a
- 局解手术学杂志 2023年32卷第5期 页码：382-388
- 作者机构：
新疆医科大学第一附属医院耳鼻喉科，新疆 乌鲁木齐 830054
- 作者简介：
阿依恒•曲库尔汗，E-mail：ayhen979@sina.com
- 基金信息：新疆维吾尔自治区区域协同创新专项项目(2020E01055)
DOI：10.11659/jjssx.10E022108
中图分类号： R739.65纸质出版日期：2023-05-25，
收稿日期：2022-10-21，
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张波,王松,阿依恒·曲库尔汗 .lncRNA NEAT1通过抑制miR-let-7a激活BZW2促进喉乳头状瘤细胞的增殖和凋亡逃逸[J].局解手术学杂志,2023,32(05):382-388.
ZHANG Bo,WANG Song,Ayiheng·Qukuerhan .lncRNA NEAT1 promoting the proliferation and apoptosis escape of laryngeal papilloma cells by activating BZW2 through the inhibition of miR-let-7a[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(05):382-388.
张波,王松,阿依恒·曲库尔汗 .lncRNA NEAT1通过抑制miR-let-7a激活BZW2促进喉乳头状瘤细胞的增殖和凋亡逃逸[J].局解手术学杂志,2023,32(05):382-388. DOI： 10.11659/jjssx.10E022108.
ZHANG Bo,WANG Song,Ayiheng·Qukuerhan .lncRNA NEAT1 promoting the proliferation and apoptosis escape of laryngeal papilloma cells by activating BZW2 through the inhibition of miR-let-7a[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(05):382-388. DOI： 10.11659/jjssx.10E022108.
摘要
目的
2
探讨长链非编码RNA核富集转录本1（lncRNA NEAT1）对喉乳头状瘤（LP）细胞的增殖和凋亡逃逸的调控作用和机制。
方法
2
培养人喉乳头状瘤细胞Hs840.T，将正常人口腔上皮细胞（HOECs）作为对照。将Hs840.T分为lncRNA NEAT1过表达载体（pcDNA-NEAT1）组、pcDNA-NEAT1阴性对照（pcDNA-NC）组、沉默lncRNA NEAT1的小干扰RNA载体（siNEAT1）组、siNEAT1的阴性对照（siNC）组、miR-let-7a的模拟物（mimic）组、mimic阴性对照（mimic-NC）组、miR-let-7a抑制剂（inhibitor）组、inhibitor阴性对照（inhibitor-NC）组、沉默碱性亮氨酸拉链和W2结构域2（BZW2）的小干扰RNA载体（siBZW2）组、siBZW2的阴性对照（siBZW2-NC）组、pcDNA-NEAT1联合siBZW2给药（pcDNA-NEAT1+siBZW2）组、pcDNA-NEAT1联合siBZW2-NC给药（pcDNA-NEAT1+siBZW2-NC）组。qRT-PCR检测各组中lncRNA NEAT1及其预测靶分子miR-let-7a的表达。Western blot和qRT-PCR检测BZW2的表达。双荧光素酶报告基因实验检测Hs840.T中lncRNA NEAT1和miR-let-7a以及miR-let-7a和BZW2的靶向关系。MTT实验测定Hs840.T的增殖能力。Annexin V-FITC/PI双染法检测Hs840.T的凋亡逃逸能力。
结果
2
Hs840.T中的lncRNA NEAT1和BZW2表达高于HOECs（
P
<
0.05），而miR-let-7a表达低于HOECs（
P
<
0.05）。双荧光素酶报告基因实验结果表明，Hs840.T中lncRNA NEAT1吸附抑制miR-let-7a并上调BZW2的表达（
P
<
0.05），且BZW2是miR-let-7a的靶基因。与pcDNA-NC组相比，pcDNA-NEAT1组Hs840.T的增殖率增加（
P
<
0.05），早期、晚期凋亡率均降低（
P
<
0.05）；与siNC组相比，siNEAT1组Hs840.T的增殖率降低（
P
<
0.05），早期、晚期凋亡率均增加（
P
<
0.05）。另外，与pcDNA-NEAT1+siBZW2-NC组相比，pcDNA-NEAT1+siBZW2组Hs840.T的增殖率降低（
P
<
0.05），早期、晚期凋亡率均增高（
P
<
0.05）。
结论
2
lncRNA NEAT1通过抑制miR-let-7a激活BZW2促进LP细胞的增殖和凋亡逃逸。
Abstract
Objective
2
To investigate the regulatory effect of long non-coding RNA nuclear enriched abundant transcript 1 (lncRNA NEAT1) on the proliferation and apoptosis escape of laryngeal papilloma (LP) cells and its mechanism.
Methods
2
Human laryngeal papilloma cells Hs840.T were cultured
and the normal human oral epithelial cells (HOECs) were used as the control.Then
the Hs840.T were divided into the lncRNA NEAT1 over-expression vector (pcDNA-NEAT1) group
pcDNA-NEAT1 negative control (pcDNA-NC) group
lncRNA NEAT1 silencing small interfering RNA vector (siNEAT1) group
siNEAT1 negative control (siNC) group
mimic of miR-let-7a (mimic) group
mimic negative control (mimic-NC) group
inhibitor of miR-let-7a (inhibitor) group
inhibitor negative control (inhibitor-NC) group
basic leucine zipper and W2 domains 2 (BZW2) silencing small interfering RNA vector (siBZW2) group
siBZW2 negative control (siBZW2-NC) group
combined administration of pcDNA-NEAT1 and siBZW2 (pcDNA-NEAT1+siBZW2) group
and combined administration of pcDNA-NEAT1 and siBZW2-NC (pcDNA-NEAT1+siBZW2-NC) group.qRT-PCR was used to detect the expression of lncRNA NEAT1 and its predicted target molecule miR-let-7a in each group.Western blot and qRT-PCR were used to detect the expression of BZW2.Dual-luciferase reporter gene assay was used to investigate the targeting relationship between lncRNA NEAT1 and miR-let-7a and the targeting relationship between miR-let-7a and BZW2 in Hs840.T.MTT assay was used to determine the proliferation ability of Hs840.T.Annexin V-FITC/PI double staining assay was used to determine the apoptosis escape ability of Hs840.T.
Results
2
The expression of lncRNA NEAT1 and BZW2 in Hs840.T were higher than those in HOECs (
P
<
0.05)
while the expression of miR-let-7a in Hs840.T was lower than that in HOECs (
P
<
0.05).The results of dual-luciferase reporter gene assay showed that lncRNA NEAT1 sponged and inhibited miR-let-7a
thereby up regulating the expression of BZW2 in Hs840.T (
P
<
0.05)
and BZW2 was a target gene of miR-let-7a.Compared with the pcDNA-NC group
the pcDNA-NEAT1 group showed a higher proliferation rate of Hs840.T(
P
<
0.05)
and lower early and later apoptosis rates (
P
<
0.05);compared with the siNC group
the siNEAT1 group showed a lower proliferation rate of Hs840.T (
P
<
0.05)
and higher early and later apoptosis rates (
P
<
0.05).In addition
compared with the pcDNA-NEAT1+siBZW2-NC group
the pcDNA-NEAT1+siBZW2 group showed a lower proliferation rate of Hs840.T (
P
<
0.05)
and higher early and later apoptosis rates (
P
<
0.05).
Conclusion
2
lncRNA NEAT1 promotes the proliferation and apoptosis escape of LP cells by activating BZW2 through the inhibition of miR-let-7a.
关键词
喉乳头状瘤长链非编码RNA核富集转录本1miR-let-7a碱性亮氨酸拉链和W2结构域2细胞凋亡细胞增殖
Keywords
laryngeal papillomalong non-coding RNA nuclear enriched abundant transcript 1miR-let-7abasic leucine zipper and W2 domains 2cell apoptosiscell proliferation
references
Kurita T，Chitose S，Sato K，et al.Pathological mechanisms of laryngeal papillomatosis based on laryngeal epithelial characteristics［J］.Laryngoscope Investig Otolaryngol，2019，4（1）：89-94.doi：10.1002/lio2.242http://dx.doi.org/10.1002/lio2.242.
Matsuzaki H，Makiyama K，Hirai R，et al.Multi‐year effect of human papillomavirus vaccination on recurrent respiratory papillomatosis［J］.Laryngoscope，2020，130（2）：442-447.doi：10.1002/lary.27993http://dx.doi.org/10.1002/lary.27993.
Ouda AM，Elsabagh AA，Elmakaty IM，et al.HPV and recurrent respiratory papillomatosis：a brief review［J］.Life，2021，11（11）：1279-1285.doi：10.3390/life11111279http://dx.doi.org/10.3390/life11111279.
Fernandes JCR，Acuña SM，Aoki JI，et al.Long non-coding RNAs in the regulation of gene expression：physiology and disease［J］.Noncoding RNA，2019，5（1）：17-23.doi：10.3390/ncrna5010017http://dx.doi.org/10.3390/ncrna5010017.
Wang L，Ren C，Xu Y，et al.The LINC00922 aggravates ovarian cancer progression via sponging miR-361-3p［J］.J Ovarian Res，2021，14：77.doi：10.1186/s13048-021-00828-7http://dx.doi.org/10.1186/s13048-021-00828-7.
Dong H，Hu J，Zou K，et al.Correction to：activation of LncRNA TINCR by H3K27 acetylation promotes Trastuzumab resistance and epithelial-mesenchymal transition by targeting MicroRNA-125b in breast cancer［J］.Mol Cancer，2021，20（1）：87.doi：10.1186/s12943-021-01385-9http://dx.doi.org/10.1186/s12943-021-01385-9.
Su Z，Chen M，Ding R，et al.Long noncoding RNA HCG11 suppresses the malignant phenotype of nonsmall cell lung cancer cells by targeting a miR875/SATB2 axis［J］.Mol Med Rep，2021，24（2）：552.doi：10.3892/mmr.2021.12191http://dx.doi.org/10.3892/mmr.2021.12191.
Han L，Zheng C，Wu S.Long noncoding RNA NEAT1 promotes the malignancy of laryngeal squamous cell carcinoma by regulating the microRNA2045p/SEMA4B axis［J］.Oncol Lett，2021，22（5）：1-16.doi：10.3892/ol.2021.13063http://dx.doi.org/10.3892/ol.2021.13063.
Shishodia G，Verma G，Srivastava Y，et al.Deregulation of microRNAs Let-7a and miR-21 mediate aberrant STAT3 signaling during human papillomavirus-induced cervical carcinogenesis：role of E6 oncoprotein［J］.BMC Cancer，2014，23（14）：996.doi：10.1186/1471-2407-14-996http://dx.doi.org/10.1186/1471-2407-14-996.
Liu L，Zhao J，Peng Y，et al.MiR-let-7a-5p inhibits invasion and migration of hepatoma cells by regulating BZW2 expression ［J］.Onco Targets Ther，2020，13（1）：12269-12279.doi：10.2147/OTT.S278954http://dx.doi.org/10.2147/OTT.S278954.
Geng F，Jia WC，Li T，et al.Knockdown of lncRNA NEAT1 suppresses proliferation and migration，and induces apoptosis of cervical cancer cells by regulating the miR377/FGFR1 axis［J］.Mol Med Rep，2022，25（1）：10.doi：10.3892/mmr.2021.12526http://dx.doi.org/10.3892/mmr.2021.12526.
Zhao D，Hou Y.Long non-coding RNA nuclear-enriched abundant transcript 1 （LncRNA NEAT1） upregulates Cyclin T2 （CCNT2） in laryngeal papilloma through sponging miR-577/miR-1224-5p and blocking cell apoptosis［J］.Bioengineered，2022，13（1）：1828-1837.doi：10.1080/21655979.2021.2017653http://dx.doi.org/10.1080/21655979.2021.2017653.
Liu J，Yang T，Zhang Y，et al.Promotion of BZW2 by LINC00174 through miR-4500 inhibition enhances proliferation and apoptosis evasion in laryngeal papilloma［J］.Cancer Cell Int，2020，20（1）：471.doi：10.1186/s12935-020-01559-3http://dx.doi.org/10.1186/s12935-020-01559-3.
Fan JT，Zhou ZY，Luo YL，et al.Exosomal lncRNA NEAT1 from cancer-associated fibroblasts facilitates endometrial cancer progression via miR-26a/b-5p-mediated STAT3/YKL-40 signaling pathway［J］.Neoplasia，2021，23（7）：692-703.doi：10.1016/j.neo.2021.05.004http://dx.doi.org/10.1016/j.neo.2021.05.004.
Shen X，Ye Z，Wu W，et al.lncRNA NEAT1 facilitates the progression of colorectal cancer via the KDM5A/Cul4A and Wnt signaling pathway［J］.Int J Oncol，2021，59（1）：51.doi：10.3892/ijo.2021.5231http://dx.doi.org/10.3892/ijo.2021.5231.
Qiao Y，Jin T，Guan S，et al.Long non-coding RNA Lnc-408 promotes invasion and metastasis of breast cancer cell by regulating LIMK1［J］.Oncogene，2021，40（24）：4198-4213.doi：10.1038/s41388-021-01845-yhttp://dx.doi.org/10.1038/s41388-021-01845-y.
Yu T，Yu J，Lu L，et al.MT1JP-mediated miR-24-3p/BCL2L2 axis promotes Lenvatinib resistance in hepatocellular carcinoma cells by inhibiting apoptosis［J］.Cell Oncol （Dordr），2021，44（4）：821-834.doi：10.1007/s13402-021-00605-0http://dx.doi.org/10.1007/s13402-021-00605-0.
Duan YR，Chen BP，Chen F，et al.LncRNA lnc-ISG20 promotes renal fibrosis in diabetic nephropathy by inducing AKT phosphorylation through miR-486-5p/NFAT5［J］.J Cell Mol Med，2021，25（11）：4922-4937.doi：10.1111/jcmm.1628http://dx.doi.org/10.1111/jcmm.1628.
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