兔耳缘交替静脉滴注胺碘酮致血管损伤及修复的实验研究

潘婉; 郑蓉; 祝炜; 王莉

doi:10.11659/jjssx.09E022149

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兔耳缘交替静脉滴注胺碘酮致血管损伤及修复的实验研究

基础研究 | 更新时间：2023-11-24

- 兔耳缘交替静脉滴注胺碘酮致血管损伤及修复的实验研究
- Experimental study on vascular injury and repair induced by alternate intravenous instillation of amiodarone in rabbits marginal ear vein
- 局解手术学杂志 2023年32卷第11期页码：974-978
- 作者机构：
  
  武汉市第一医院心血管内科，湖北武汉 430000
- 作者简介：
  
  王莉，E-mail：Wangli99@163.com
- 基金信息：
  
  湖北省自然科学基金(2019CFC902)
- DOI：10.11659/jjssx.09E022149
  中图分类号： R339.16
- 纸质出版日期：2023-11-25，
  
  收稿日期：2022-09-29，
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潘婉,郑蓉,祝炜等.兔耳缘交替静脉滴注胺碘酮致血管损伤及修复的实验研究[J].局解手术学杂志,2023,32(11):974-978.

PAN Wan,ZHENG Rong,ZHU Wei,et al.Experimental study on vascular injury and repair induced by alternate intravenous instillation of amiodarone in rabbits marginal ear vein[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(11):974-978.
潘婉,郑蓉,祝炜等.兔耳缘交替静脉滴注胺碘酮致血管损伤及修复的实验研究[J].局解手术学杂志,2023,32(11):974-978. DOI： 10.11659/jjssx.09E022149.

PAN Wan,ZHENG Rong,ZHU Wei,et al.Experimental study on vascular injury and repair induced by alternate intravenous instillation of amiodarone in rabbits marginal ear vein[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(11):974-978. DOI： 10.11659/jjssx.09E022149.

摘要

目的

探究兔耳缘交替静脉滴注胺碘酮对血管损伤和修复的影响。

方法

取90只大耳白兔为实验对象，随机分为A组、B组和C组，每组30只。A组在滴注完毕后立即采集标本，B组滴注完毕后72 h收集标本，C组滴注完毕后1周收集样本。再将每组进一步随机分为6个亚组，即1 h组、2 h组、3 h组、4 h组、5 h组、6 h组，每组5只，并分别每1 h、2 h、3 h、4 h、5 h、6 h更换到另一侧耳缘静脉通道给药。肉眼观察兔耳缘静脉炎程度；ELISA检测C反应蛋白（CRP）浓度；HE染色检测炎症程度；TUNEL染色检测血管内皮细胞凋亡水平。

结果

A组中1~4 h组大耳白兔耳缘静脉均无明显肉眼可见的变化，5 h组1只大耳白兔输液部位红肿，6 h组1只大耳白兔出现血管轻微条索样改变。A组中1 h、2 h、3 h组CRP浓度比较差异无统计学意义（

0.05）；4 h、5 h、6 h组CRP浓度逐渐增加（

0.05）。HE染色和TUNEL染色结果显示，A组4 h内血管炎症损伤程度最小，4 h开始炎症损伤逐渐增加，血管内皮细胞凋亡也明显增加；B组经72 h修复后血管炎症程度和细胞凋亡改善；C组经1周修复后血管炎症程度和细胞凋亡进一步改善。

结论

交替静脉滴注胺碘酮在4 h内对静脉损伤的影响最小，兔耳缘血管内皮细胞的凋亡程度最轻，因此推荐最佳交替时间为4 h内。

Abstract

Objective

To explore the effects of alternate intravenous instillation of amiodarone on the Vascular injury and repair of rabbit marrginal ear vein.

Methods

Ninety large-eared white rabbits were selected as experimental subjects and randomly divided into groups A， B and C， with 30 rabbits in each group. The samples of group A were collected immediately after the completion of the infusion， the samples of group B were collected 72 hours after the completion of the infusion，and the samples of group C were collected 1 week after the completion of the infusion. Each group was further randomly divided into 6 subgroups，namely，1-hour group，2-hour group，3-hour group，4-hour group，5-hour group and 6-hour group，with 5 rabbits in each group，and amiodarone was instilled into the other side of the margin ear vein every 1 hour，2 hours，3 hours， 4 hours， 5 hours and 6 hours.The degree of phlebitis of ear margin was observed. ELISA was used to detected the C-reactive protein （CRP） concentration.HE staining was used to determine the degree of inflammation.TUNEL was used to detect the apoptosis level of vascular endothelial cells.

Results

In group A， there was no visible change in the margin ear vein of rabbit in the 1- to 4-hour groups； the infusion site of 1 large-eared white rabbit in 5-hour group was red and swollen， while the blood vessel of 1 large-eared white rabbit in 6-hour group was slightly stringlike. The was no statistically significant difference in C-reactive protein （CRP） concentration among the 1 h， 2 h， and 3 h groups of group A （

0.05）；and the CRP concentrations gradually increased in the 4-hour， 5-hour， and 6-hour groups （

0.05）. The results of HE staining and TUNEL staining showed that the vascular inflammatory injury was minimal within 4 hours in group A，while the inflammatory injury gradually aggravated from 4 hours onwards，and the endothelial cell apoptosis also significantly increased.In group B， injury inflammatory and cell apoptosis were improved after 72 hours of repair. In group C， the degree of vascular inflammatory injury and cell apoptosis further improved after 1 week of repair.

Conclusion

Alternate intravenous instillation of amiodarone has the least injury to the vein within 4 hours，and the lightest degree of apoptosis of endothelial cells in rabbit margin ear vein；therefore，the best alternate time is recommended to be within 4 hours.

关键词

交替静脉滴注胺碘酮血管内皮细胞凋亡修复静脉炎血管损伤

Keywords

alternate intravenous instillationamiodaroneapoptosis of vascular endothelial cellsrepairphlebitisvascular injury

references

Mailhe M，Aubry C，Brouqui P，et al.Complications of peripheral venous catheters （PVCs）：the need to propose alternative route of administration［J］.Int J Antimicrob Agents，2020，55（3）：105875.doi：10.1016/j.ijantimicag.2020.105875http://dx.doi.org/10.1016/j.ijantimicag.2020.105875.

朱蓓，马曾庆，马梦圆，等.Ⅰ期药物临床试验中静脉输液常见问题及对策［J］.中国药业，2020，29（12）：35-37.doi：10.3969/j.issn.1006-4931.2020.12.009http://dx.doi.org/10.3969/j.issn.1006-4931.2020.12.009.

尹春红.交替静脉输注护理干预对甘露醇所致静脉炎发生率的影响［J］.实用临床医药杂志，2014，18（2）：75-76.doi：10.7619/jcmp.201402027http://dx.doi.org/10.7619/jcmp.201402027.

庄惠人.酚妥拉明加林可霉素利多卡因凝胶处理胺碘酮输注外渗致静脉炎的动物实验研究［J］.中华现代护理杂志，2018，24（17）：2036-2039.doi：10.3760/cma.j.issn.1674-2907.2018.17.011http://dx.doi.org/10.3760/cma.j.issn.1674-2907.2018.17.011.

Gorski LA. The 2016 infusion therapy standards of practice［J］ .Home Healthc Now， 2017， 35（1）： 10-18.doi： 10.1097/NHH. 0000000000000481http://dx.doi.org/10.1097/NHH.0000000000000481.

张岚，周利娟.大黄复方膏外敷预防留置针静脉治疗所致机械性静脉炎的实验研究［J］.黑龙江医药，2019，32（4）：777-779.doi：10.14035/j.cnki.hljyy.2019.04.008http://dx.doi.org/10.14035/j.cnki.hljyy.2019.04.008.

李雅，赵文萍，冯惠平，等.胺碘酮联合厄贝沙坦治疗慢性心力衰竭合并心律失常的临床效果［J］.中国医药，2020，15（1）：26-29.doi：10.3760/j.issn.1673-4777.2020.01.007http://dx.doi.org/10.3760/j.issn.1673-4777.2020.01.007.

王莉，郑蓉，陈满满，等.胺碘酮注射液致外周静脉损害的预防性护理方案构建与应用［J］.广西医学，2022，44（18）：2196-2200.doi：10.11675/j.issn.0253-4304.2022.18.28http://dx.doi.org/10.11675/j.issn.0253-4304.2022.18.28.

覃翠敏.胺碘酮不同给药方式对静脉炎症的影响——评《临床检验医学案例分析》［J］.检验医学，2020，35（3）：290.doi：10.3969/j.issn.1673-8640.2020.03.024http://dx.doi.org/10.3969/j.issn.1673-8640.2020.03.024.

孙杰，廖菊英，谢思敏，等.神经科患者外周静脉双通道交替使用对防范静脉外渗的效果观察［J］.中西医结合心血管病电子杂志，2020，8（28）：132-133.doi：10.16282/j.cnki.cn11-9336/r.2020.28.091http://dx.doi.org/10.16282/j.cnki.cn11-9336/r.2020.28.091.

曹利芸.穿刺部位及方法对胺碘酮致静脉炎的影响［J］.西部医学，2011，23（2）：380-381. doi：10.3969/j.issn.1672-3511. 2011.02.078http://dx.doi.org/10.3969/j.issn.1672-3511.2011.02.078.

Chiu PC，Lee YH，Hsu HT，et al.Establish a perioperative check forum for peripheral intravenous access to prevent the occurrence of phlebitis［J］.Kaohsiung J Med Sci，2015，31（4）：215-221.doi：10.1016/j.kjms.2015.01.007http://dx.doi.org/10.1016/j.kjms.2015.01.007.

王英，李福珍，朱海云.阿奇霉素对支原体肺炎患儿血栓性静脉炎发生的影响及临床护理［J］.血栓与止血学，2019，25（5）：873-875.doi：10.3969/j.issn.1009-6213.2019.05.061http://dx.doi.org/10.3969/j.issn.1009-6213.2019.05.061.

杨雪佳，胡靖，张媛媛，等.盐酸法舒地尔对PCI术后慢血流冠心病患者内皮细胞损伤的作用及其机制研究［J］.中国现代医学杂志，2020，30（13）：31-36.doi：10.3969/j.issn.1005-8982. 2020.13.007http://dx.doi.org/10.3969/j.issn.1005-8982.2020.13.007.

闫鲜艳，郭锦丽，赵清丽，等.经外周输注胺碘酮与乐凡命导致静脉炎极限时间实验研究［J］.护理研究，2020，34（8）：1356-1360.doi：10.12102/j.issn.1009-6493.2020.08.010http://dx.doi.org/10.12102/j.issn.1009-6493.2020.08.010.

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