感染性心内膜炎细胞因子和降钙素原的表达

王若昕; 付亮; 赵金龙; 陈宗辉; 倪寅凯; 励峰

doi:10.11659/jjssx.08E023079

您当前的位置：

首页 >

文章列表页 >

感染性心内膜炎细胞因子和降钙素原的表达

临床研究 | 更新时间：2024-01-22

- 感染性心内膜炎细胞因子和降钙素原的表达
- Expressions of cytokines and procalcitonin in infective endocarditis
- 局解手术学杂志 2024年33卷第1期页码：55-58
- 作者机构：
  
  上海市第六人民医院心血管外科，上海 200233
- 作者简介：
  
  励峰，E-mail：lifeng56613@163.com
- 基金信息：
  
  上海市第六人民医院医疗集团科研基金
- DOI：10.11659/jjssx.08E023079
  中图分类号： R654.2
- 纸质出版日期：2024-01-25，
  
  收稿日期：2023-08-20，
扫描看全文
王若昕,付亮,赵金龙等.感染性心内膜炎细胞因子和降钙素原的表达[J].局解手术学杂志,2024,33(01):55-58.

WANG Ruo-xin,FU Liang,ZHAO Jin-long,et al.Expressions of cytokines and procalcitonin in infective endocarditis[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2024,33(01):55-58.
王若昕,付亮,赵金龙等.感染性心内膜炎细胞因子和降钙素原的表达[J].局解手术学杂志,2024,33(01):55-58. DOI： 10.11659/jjssx.08E023079.

WANG Ruo-xin,FU Liang,ZHAO Jin-long,et al.Expressions of cytokines and procalcitonin in infective endocarditis[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2024,33(01):55-58. DOI： 10.11659/jjssx.08E023079.

摘要

目的

探讨感染性心内膜炎（IE）患者中12项细胞因子（IL-1β、IL-2、IL-4、IL-5、IL-6、IL-8、IL-10、IL-12p70、IL-17、IFN-α、IFN-γ、TNF-α）及降钙素原的表达。

方法

纳入我院2021年12月至2022年12月IE患者10例为IE组，随机选择同期入院手术的非感染性、非风湿性瓣膜病的心内膜炎患者10例为对照组，患者均于入院时采血。采用流式细胞术检测12种细胞因子的表达及血常规指标，采用ELISA法检测降钙素原水平，采用

Pearson

法分析IE患者细胞因子表达水平间的相关性，

Spearman

法分析IE患者IL-8水平、白细胞计数与降钙素原的相关性。

结果

与对照组比较，IE组患者中细胞因子IL-1β、IL-2、IL-6、IL-10、TNF-α、IFN-α、IFN-γ、IL-12p70显著升高（

0.05），白细胞计数、中性粒细胞百分比、降钙素原水平显著升高（

0.05）。2组患者单核细胞百分比比较，差异无统计学意义（

0.05）。IE组患者中的IFN-α与IL-2、TNF-α、IL-1β、IL-12p70呈正相关；IL-2与TNF-α、IL-1β呈正相关；IL-12p70与IFN-γ呈正相关；降钙素原与IL-8、白细胞计数呈显著正相关，差异均有统计学意义（

0.05）。

结论

IE患者中IL-1β、IL-2、IL-6、IL-10、TNF-α、IFN-α、IFN-γ、IL-12p70、降钙素原等水平明显高于正常人群，这些指标的检测对于IE的早期诊断及病情严重程度的评估具有指导意义。

Abstract

Objective

To investigate the expressions of 12 cytokines （IL-1β， IL-2， IL-4， IL-5， IL-6， IL-8， IL-10， IL-12p70， IL-17， IFN-α， IFN-γ， TNF-α） and procalcitonin in patients with infective endocarditis （IE）.

Methods

Ten IE patients admitted to our hospital from December 2021 to December 2022 were included into the IE group，10 patients with non-infectious and non-rheumatic valvular diseases who were admitted to our hospital at the same period were randomly selected as the control group， and blood sampling of all patients were conducted at admission. The expressions of 12 cytokines and blood routine indexes were detected by flow cytometry， and the level of procalcitonin was detected by ELISA. The correlations among the expression levels of cytokines in IE patients were analyzed by

Pearson

method and the correlations of IL-8 level and white blood cell count with procalcitonin in IE patients were analyzed by

Spearman

method.

Results

Compared with the control group，the levels of cytokines of IL-1β，IL-2，IL-6，IL-10，TNF-α，IFN-α，IFN-γ and IL-12p70 in the IE group were significantly increased （

0.05）， the white blood cell count，neutrophil percentage and procalcitonin were significantly increased （

0.05）. There was no significant difference in the percentage of monocytes between the two groups（

0.05）.IFN-α of IE patients was positively correlated with IL-2，TNF-α，IL-1β and IL-12p70，IL-2 was positively correlated with TNF-α and IL-1β，IL-12p70 was positively correlated with IFN-γ， and procalcitonin was significantly positively correlated with IL-8 and white blood cell count， with statistically significant differences （

0.05）.

Conclusion

The levels of IL-1β， IL-2， IL-6， IL-10， TNF-α， IFN-α， IFN-γ， IL-12p70 and procalcitonin in IE patients are significantly higher than those in the normal population， and the detections of these indicators are of guiding significance for the early diagnosis of IE and the evaluation of the severity of the disease.

关键词

感染性心内膜炎细胞因子降钙素原心脏瓣膜病血常规检查

Keywords

infective endocarditiscytokinesprocalcitoninvalvular heart diseaseblood routine examination

references

Ortega-Loubon C，Muñoz-Moreno MF，García IA，et al.Nosocomial vs.community-acquired infective endocarditis in Spain：location，trends，clinical presentation，etiology，and survival in the 21st century［J］.J Clin Med，2019，8：1755.doi：10.3390/jcm8101755http://dx.doi.org/10.3390/jcm8101755.

Pant S，Patel NJ，Deshmukh A，et al.Trends in infective endocarditis incidence，microbiology，and valve replacement in the United States from 2000 to 2011［J］.J Am Coll Cardiol，2015，65：2070-2076.doi：10.1016/j.jacc.2015.03.518http://dx.doi.org/10.1016/j.jacc.2015.03.518.

Thornhill MH，Jones S，Prendergast B，et al.Quantifying infective endocarditis risk in patients with predisposing cardiac conditions［J］.Eur Heart J，2018，39：586-595.doi：10.1093/eurheartj/ehx655http://dx.doi.org/10.1093/eurheartj/ehx655.

Mercier RC，Dietz RM，Mazzola JL，et al.Beneficial influence of platelets on antibiotic efficacy in an in vitro model of Staphylococcus aureus-induced endocarditis［J］.Antimicrob Agents Chemother，2004，48（7）：2551-2557.doi：10.1128/AAC.48.7.2551-2557.2004http://dx.doi.org/10.1128/AAC.48.7.2551-2557.2004.

Deftos LJ，Roos BA，Parthemore JG.Calcium and skeletal metabolism［J］.West J Med，1975，123（6）：447-458.

向怒涛，申永.降钙素原在抗感染治疗中的动态变化研究［J］.局解手术学杂志，2015，24（4）：443-444.doi：10.11659/jjssx.1672-5042.05E015032http://dx.doi.org/10.11659/jjssx.1672-5042.05E015032.

Knudsen JB，Fuursted K，Petersen E，et al.Procalcitonin in 759 patients clinically suspected of infective endocarditis［J］.Am J Med，2010，123（12）：1121-1127.doi：10.1016/j.amjmed.2010.07.018http://dx.doi.org/10.1016/j.amjmed.2010.07.018.

Mueller C，Huber P，Laifer G，et al.Procalcitonin and the early diagnosis of infective endocarditis［J］.Circulation，2004，109（14）：1707-1710.doi：10.1161/01.cir.0000126281.52345.52http://dx.doi.org/10.1161/01.cir.0000126281.52345.52.

Buyykasyk NS，Ileri M，Apler A，et al.Increased blood coagulation and platelet activation in patients with infective endocarditis and embolic events［J］.Clin Cardiol，2004，27（3）：154-158.doi：10.1002/clc.4960270312http://dx.doi.org/10.1002/clc.4960270312.

王家瑞，何金，王伟，等.改良VSD方案对烧伤早期创面修复基质金属蛋白及相关细胞因子表达的影响［J］.局解手术学杂志，2020，29（9）：721-726.doi：10.11659/jjssx.06E020008http://dx.doi.org/10.11659/jjssx.06E020008.

Declercq J，Van Damme KFA，De Leeuw E，et al.Effect of anti-interleukin drugs in patients with COVID-19 and signs of cytokine release syndrome（COV-AID）：a factorial，randomised，controlled trial［J］.Lancet Respir Med，2021，9（12）：1427-1438.doi：10.1016/S2213-2600（21）00377-5http://dx.doi.org/10.1016/S2213-2600（21）00377-5.

Gao S，Jiang J，Jin C，et al.Interleukin-8 as a candidate for thymoma identification and recurrence surveillance［J］.Nat Commun，2020，11（1）：4881.doi：10.1038/s41467-020-18697-xhttp://dx.doi.org/10.1038/s41467-020-18697-x.

Schalper KA，Carleton M，Zhou M，et al.Elevated serum interleukin-8 is associated with enhanced intratumor neutrophils and reduced clinical benefit of immune-checkpoint inhibitors［J］.Nat Med，2020，26（5）：688-692.doi：10.1038/s41591-020-0856-xhttp://dx.doi.org/10.1038/s41591-020-0856-x.

Satoshi N，Kotaro M，Sotaro H，et al.Recent picture of infective endocarditis in Japan--lessons from cardiac disease registration （CADRE-IE）［J］.Circ J，2013，77：1558-1564.doi：10.1253/circj.cj-12-1101http://dx.doi.org/10.1253/circj.cj-12-1101.

Thuny F，Grisoli D，Collart F，et al.Management of infective endocarditis：challenges and perspectives［J］.Lancet，2012，379（9819）：965-975.doi：10.1016/S0140-6736（11）60755-1http://dx.doi.org/10.1016/S0140-6736（11）60755-1.

Tattevin P，Watt G，Revest M，et al.Update on blood culture-negative endocarditis［J］.Med Mal Infect，2015，45（1/2）：1-8.doi：10.1016/j.medmal.2014.11.003http://dx.doi.org/10.1016/j.medmal.2014.11.003.

Karzai W，Oberhoffer M，Meier-Hellmann A，et al.Procalcitonin--a new indicator of the systemic response to severe infections［J］.Infection，1997，25（6）：329-334.doi：10.1007/BF01740811http://dx.doi.org/10.1007/BF01740811.

Tanriverdi H，Örnek T，Erboy F，et al.Comparison of diagnostic values of procalcitonin，C-reactive protein and blood neutrophil/lymphocyte ratio levels in predicting bacterial infection in hospitalized patients with acute exacerbations of COPD［J］.Wien Klin Wochenschr，2015，127（19/20）：756-763.doi：10.1007/s00508-014-0690-6http://dx.doi.org/10.1007/s00508-014-0690-6.

Jereb M，Kotar T，Jurca T，et al.Usefulness of procalcitonin for diagnosis of infective endocarditis［J］.Intern Emerg Med，2009，4（3）：221-226.doi：10.1007/s11739-009-0245-4http://dx.doi.org/10.1007/s11739-009-0245-4.

Konrad R，Michael M，Frank MB.Markers for sepsis diagnosis：what is useful？［J］.Crit Care Clin，2006，22（3）：503-519.doi：10.1016/j.ccc.2006.03.003http://dx.doi.org/10.1016/j.ccc.2006.03.003.

张亮达，戴建强，陈坤，等.胸腺肽α1对经口寰枢椎复位内固定术后医院获得性肺炎患者CRP、PCT及免疫细胞亚群的影响［J］.局解手术学杂志，2020，29（11）：875-878.doi：10.11659/jjssx.04E020080http://dx.doi.org/10.11659/jjssx.04E020080.

中国医药教育协会感染疾病专业委员会.感染相关生物标志物临床意义解读专家共识［J］.中华结核和呼吸杂志，2017，40（4）：243-257.doi：10.3760/cma.j.issn.1001-0939.2017.04.002http://dx.doi.org/10.3760/cma.j.issn.1001-0939.2017.04.002.

Chokkalingam V，Tel J，Wimmers F，et al.Probing cellular heterogeneity in cytokine-secreting immune cells using droplet-based microfluidics［J］.Lab Chip，2023，13（24）：4740-4744.doi：10.1039/c3lc50945ahttp://dx.doi.org/10.1039/c3lc50945a.

Carpenter LR，Moy JN，Roebuck KA.Respiratory syncytial virus and TNF alpha induction of chemokine gene expression involves differential activation of Rel A and NF-kappa B1［J］.BMC Infect Dis，2002，2：5.doi：10.1186/1471-2334-2-5http://dx.doi.org/10.1186/1471-2334-2-5.

Tian B，Nowak DE，Brasier AR，et al.A TNF-induced gene expression program under oscillatory NF-kappaB control［J］.BMC Genomics，2005，6：137.doi：10.1186/1471-2164-6-137http://dx.doi.org/10.1186/1471-2164-6-137.

Kany S，Vollrath JT，Relja B.Cytokines in inflammatory disease［J］.Int J Mol Sci，2019，20（23）：6008.doi：10.3390/ijms20236008http://dx.doi.org/10.3390/ijms20236008.

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

感染性心内膜炎的诊断与治疗进展

Luminex液态芯片技术在克罗恩病诊断与病情监测中的应用