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CDCA3在上皮性卵巢癌中的表达及意义
Expression and significance of CDCA3 in epithelial ovarian cancer
- 局解手术学杂志 2023年32卷第7期 页码：573-577
- 作者机构：
秦皇岛市第一医院妇科，河北 秦皇岛 066000
- 作者简介：
龚姗，E-mail：gsmeimei201@163.com
- 基金信息：2022年度河北省医学科学研究课题计划(20221606);秦皇岛市科学技术局(202101A066)
DOI：10.11659/jjssx.08E022172
中图分类号： R737.33纸质出版日期：2023-07-25，
收稿日期：2022-08-31，
扫 描 看 全 文
李苗,金海红,付静静等.CDCA3在上皮性卵巢癌中的表达及意义[J].局解手术学杂志,2023,32(07):573-577.
LI Miao,JIN Hai-hong,FU Jing-jing,et al.Expression and significance of CDCA3 in epithelial ovarian cancer[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(07):573-577.
李苗,金海红,付静静等.CDCA3在上皮性卵巢癌中的表达及意义[J].局解手术学杂志,2023,32(07):573-577. DOI： 10.11659/jjssx.08E022172.
LI Miao,JIN Hai-hong,FU Jing-jing,et al.Expression and significance of CDCA3 in epithelial ovarian cancer[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(07):573-577. DOI： 10.11659/jjssx.08E022172.
摘要
目的
2
探讨细胞分裂周期相关蛋白3（CDCA3）在上皮性卵巢癌中的表达及其对癌细胞增殖、侵袭和迁移的影响。
方法
2
收集于本院进行手术切除的50例患者的卵巢癌组织和30例患者的正常卵巢组织，选取A2780、Hey、CAOV3、SKOV3细胞进行研究。RT-PCR检测组织和细胞中CDCA3 mRNA表达水平，Western blot检测组织和细胞中CDCA3蛋白表达水平。将CAOV3细胞分为沉默组和Control组，沉默组转染CDCA3 siRNA，Control组转染阴性对照序列；将Hey细胞分为过表达组和对照组，过表达组用CAOV3慢病毒感染，对照组用空载体慢病毒感染。CCK-8检测细胞活性；Transwell实验检测细胞侵袭和迁移。
结果
2
与正常卵巢组织比较，上皮性卵巢癌组织中CDCA3 mRNA和蛋白表达水平较高（
P
<
0.05）；在A2780、Hey、CAOV3、SKOV3上皮性卵巢癌细胞系中，CAOV3 mRNA和蛋白表达水平最高，而Hey最低。沉默CDCA3后CAOV3细胞CDCA3蛋白表达水平、细胞增殖活力、侵袭和迁移能力降低（
P
<
0.05），Cyclin D1、MMP-9蛋白表达水平降低（
P
<
0.05），而E-cadherin蛋白表达水平升高（
P
<
0.05）。过表达CDCA3后Hey细胞CDCA3蛋白表达水平、细胞增殖活力、侵袭和迁移能力升高（
P
<
0.05），Cyclin D1、MMP-9蛋白表达水平升高（
P
<
0.05），而E-cadherin蛋白表达水平降低（
P
<
0.05）。
结论
2
CDCA3在上皮性卵巢癌中高表达，能够促进上皮性卵巢癌细胞的增殖、侵袭和迁移。
Abstract
Objective
2
To investigate the expression of cell division cycle associated protein 3 （CDCA3） in epithelial ovarian cancer and its effect on the proliferation， invasion and migration of cancer cells.
Methods
2
The ovarian cancer tissue of 50 patients and the normal ovarian tissue of 30 patients who underwent surgical resection in our hospital were collected， and A2780， Hey， CAOV3， SKOV3 cells were selected for research. RT-PCR was used to detect the expression level of CDCA3 mRNA in tissues and cells， and Western blot was used to detect the expression level of CDCA3 protein in tissues and cells. CAOV3 cells were divided into the silence group and the Control group， the silence group was transfected with CDCA3 siRNA， while the Control group was transfected with negative control sequence. Hey cells were divided into the overexpression group and the control group， the overexpression group was infected with CAOV3 lentivirus， and the control group was infected with empty vector lentivirus. Cell viability was detected by CCK-8； and the cell invasion and migration were detected by Transwell assay.
Results
2
Compared with normal ovarian tissue， the expression levels of CDCA3 mRNA and CDCA3 protein in epithelial ovarian cancer tissue were higher （
P
<
0.05）. Among the epithelial ovarian cancer cell lines A2780， Hey， CAOV3， and SKOV3， the expression levels of CAOV3 mRNA and CDCA3 protein were the highest， while the expression levels of Hey were the lowest. After CDCA3 was silenced， the expression level of CDCA3 protein， cell proliferation activity， invasive and migration ability of CAOV3 cells decreased （
P
<
0.05）， the expression levels of Cyclin D1 and MMP-9 protein decreased （
P
<
0.05）， while the expression level of E-cadherin protein increased （
P
<
0.05）. After CDCA3 was overexpressed， the expression level of CDCA3 protein， cell proliferation activity， invasive and migration ability of Hey cells increased （
P
<
0.05）， the expression levels of Cyclin D1 and MMP-9 protein increased （
P
<
0.05）， while the expression level of E-cadherin protein decreased （
P
<
0.05）.
Conclusion
2
CDCA3 is highly expressed in epithelial ovarian cancer and can promote the proliferation， invasion and migration of epithelial ovarian cancer cells.
关键词
细胞分裂周期相关蛋白3上皮性卵巢癌增殖侵袭迁移
Keywords
cell division cycle associated protein 3epithelial ovarian cancerproliferationinvasionmigration
references
Stewart C，Ralyea C，Lockwood S.Ovarian cancer：an integrated review［J］.Semin Oncol Nurs，2019，35（2）：151-156.doi：10.1016/j.soncn. 2019.02.001http://dx.doi.org/10.1016/j.soncn.2019.02.001.
Oliveira L，Horvat N，Andrieu PIC，et al.Ovarian cancer staging：what the surgeon needs to know［J］.Br J Radiol，2021，94（1125）：20210091.doi：10.1259/bjr.20210091http://dx.doi.org/10.1259/bjr.20210091.
金福军，龙行涛.上皮性卵巢癌腹腔转移机制及治疗的研究进展［J］.重庆医学，2022，51（12）：2140-2144.doi：10.3969/j.issn.1671-8348.2022.12.034http://dx.doi.org/10.3969/j.issn.1671-8348.2022.12.034.
Wang Z，Chen S，Wang G，et al.CDCA3 is a novel prognostic biomarker associated with immune infiltration in hepatocellular carcinoma［J］.Biomed Res Int，2021，2021：6622437.doi：10.1155/2021/6622437http://dx.doi.org/10.1155/2021/6622437.
Zheng P，Wu K，Gao Z，et al.KIF4A promotes the development of bladder cancer by transcriptionally activating the expression of CDCA3［J］.Int J Mol Med，2021，47（6）：99.doi：10.3892/ijmm.2021.4932http://dx.doi.org/10.3892/ijmm.2021.4932.
Yu J，Hua R，Zhang Y，et al.DNA hypomethylation promotes invasion and metastasis of gastric cancer cells by regulating the binding of SP1 to the CDCA3 promoter［J］.J Cell Biochem，2020，121（1）：142-151.doi：10.1002/jcb.28993http://dx.doi.org/10.1002/jcb.28993.
Liu Y，Cheng G，Huang Z，et al.Long noncoding RNA SNHG12 promotes tumour progression and sunitinib resistance by upregulating CDCA3 in renal cell carcinoma［J］.Cell Death Dis，2020，11（7）：515.doi：10.1038/s41419-020-2713-8http://dx.doi.org/10.1038/s41419-020-2713-8.
Li H，Li M，Yang C，et al.Prognostic value of CDCA3 in kidney renal papillary cell carcinoma［J］.Aging （Albany NY），2021，13（23）：25466-25483.doi：10.18632/aging.203767http://dx.doi.org/10.18632/aging.203767.
Shen D，Fang Y，Zhou F，et al.The inhibitory effect of silencing CDCA3 on migration and proliferation in bladder urothelial carcinoma［J］.Cancer Cell Int，2021，21（1）：257.doi：10.1186/s12935-021-01969-xhttp://dx.doi.org/10.1186/s12935-021-01969-x.
彭志霞，贾佳，于东坡，等.miR-30a对卵巢癌细胞PTEN/PI3K/AKT/mTOR自噬通路及顺铂耐药性的影响［J］.现代肿瘤医学，2021，29（18）：3143-3148.doi：10.3969/j.issn.1672-4992. 2021.18.003http://dx.doi.org/10.3969/j.issn.1672-4992.2021.18.003.
郭莉，杨媛.PAX9在卵巢癌中的表达及对卵巢癌细胞增殖，迁移和侵袭能力的影响［J］.中国性科学，2021，23（12）：87-91.doi：10.3969/j.issn.1672-1993.2021.12.025http://dx.doi.org/10.3969/j.issn.1672-1993.2021.12.025.
Chen C，Chen S，Luo M，et al.The role of the CDCA gene family in ovarian cancer［J］.Ann Transl Med，2020，8（5）：190.doi：10.21037/atm.2020.01.99http://dx.doi.org/10.21037/atm.2020.01.99.
Sahin KB，Shah ET，Ferguson GP，et al.Elevating CDCA3 levels enhances tyrosine kinase inhibitor sensitivity in TKI-resistant EGFR mutant non-small-cell lung cancer［J］.Cancers （Basel），2021，13（18）：4651.doi：10.3390/cancers13184651http://dx.doi.org/10.3390/cancers13184651.
Zhang W，Lu Y，Li X，et al.CDCA3 promotes cell proliferation by activating the NF-κB/cyclin D1 signaling pathway in colorectal cancer［J］.Biochem Biophys Res Commun，2018，500（2）：196-203.doi：10.1016/j.bbrc.2018.04.034http://dx.doi.org/10.1016/j.bbrc.2018.04.034.
Qian W，Zhang Z，Peng W，et al.CDCA3 mediates p21-dependent proliferation by regulating E2F1 expression in colorectal cancer［J］.Int J Oncol，2018，53（5）：2021-2033.doi：10.3892/ijo.2018.4538http://dx.doi.org/10.3892/ijo.2018.4538.
王芹，孙正魁.CDCA3在肿瘤领域的研究进展［J］.实用癌症杂志，2018，33（6）：1043-1044.doi：10.3969/j.issn.1001-5930. 2018.06.053http://dx.doi.org/10.3969/j.issn.1001-5930.2018.06.053.
Montalto FI，De Amicis F.Cyclin D1 in cancer：a molecular connection for cell cycle control，adhesion and invasion in tumor and stroma［J］.Cells，2020，9（12）：2648.doi：10.3390/cells9122648http://dx.doi.org/10.3390/cells9122648.
Gobin E，Bagwell K，Wagner J，et al.A pan-cancer perspective of matrix metalloproteases （MMP） gene expression profile and their diagnostic/prognostic potential［J］.BMC Cancer，2019，19（1）：581.doi：10.1186/s12885-019-5768-0http://dx.doi.org/10.1186/s12885-019-5768-0.
Wang X，Yang B，She Y，et al.The lncRNA TP73-AS1 promotes ovarian cancer cell proliferation and metastasis via modulation of MMP2 and MMP9［J］.J Cell Biochem，2018，119（9）：7790-7799.doi：10.1002/jcb.27158http://dx.doi.org/10.1002/jcb.27158.
Shash LS，Ibrahim RA，Elgohary SA.E-cadherin and N-cadherin immunohistochemical expression in proliferating urothelial lesions：potential novel cancer predictive EMT profiles［J］.Appl Immunohistochem Mol Morphol，2021，29（9）：657-666.doi：10.1097/PAI. 0000000000000940http://dx.doi.org/10.1097/PAI.0000000000000940.
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