富勒醇促进小鼠皮肤创面愈合的疗效及机制

梁富程; 何志强; 张伟楠; 巩志丹; 范晓棠; 鲁元刚

doi:10.11659/jjssx.07E023096

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富勒醇促进小鼠皮肤创面愈合的疗效及机制

基础研究 | 更新时间：2023-10-23

- 富勒醇促进小鼠皮肤创面愈合的疗效及机制
- Efficacy and mechanism of fullerenol in promoting skin wound healing of mice
- 局解手术学杂志 2023年32卷第10期页码：870-875
- 作者机构：
  
  1.陆军军医大学大坪医院整形美容科，重庆 400042
  2.中国人民解放军31411部队，辽宁沈阳 110020
  3.陆军军医大学医学心理系军事认知心理学教研室，重庆 400038
- 作者简介：
  
  范晓棠，E-mail：fanxiaotang2005@163.com
  鲁元刚，E-mail：skin515@163.com
- 基金信息：
  
  陆军特色医学中心人才创新能力培养计划(2019CXJSC034)
- DOI：10.11659/jjssx.07E023096
  中图分类号： R62;R751.05
- 纸质出版日期：2023-10-25，
  
  收稿日期：2023-07-23，
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梁富程,何志强,张伟楠等.富勒醇促进小鼠皮肤创面愈合的疗效及机制[J].局解手术学杂志,2023,32(10):870-875.

LIANG Fu-cheng,HE Zhi-qiang,ZHANG Wei-nan,et al.Efficacy and mechanism of fullerenol in promoting skin wound healing of mice[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(10):870-875.
梁富程,何志强,张伟楠等.富勒醇促进小鼠皮肤创面愈合的疗效及机制[J].局解手术学杂志,2023,32(10):870-875. DOI： 10.11659/jjssx.07E023096.

LIANG Fu-cheng,HE Zhi-qiang,ZHANG Wei-nan,et al.Efficacy and mechanism of fullerenol in promoting skin wound healing of mice[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(10):870-875. DOI： 10.11659/jjssx.07E023096.

摘要

目的

探究生物材料富勒醇在小鼠皮肤创面愈合中的疗效及机制。

方法

将24只6~8周龄雄性C57BL/6小鼠随机分为假手术组（对照组）、模型组+生理盐水组（NS组）及模型组+富勒醇组（FUL组），每组8只。FUL组在建模后腹腔注射10 mg·kg

-1

·d

-1

富勒醇7 d，NS组及对照组注射等体积生理盐水。每日记录小鼠体质量，分别于治疗第3、7天对创面进行拍照并计算相应的创面愈合率。治疗后通过HE染色和Masson染色观察创面皮肤组织愈合情况；RT-qPCR检测创缘组织TNF-α、IL-6、IL-1β mRNA水平；Western blot检测创缘组织NF-κB、TNF-α、IL-6、IL-1β蛋白水平；ELISA检测小鼠血清TNF-α、IL-6、IL-1β及创缘组织MDA、SOD水平。

结果

各组小鼠每天的体质量无明显变化（

0.05）。第3、7天，FUL组创面愈合率明显高于NS组（

0.05）；与NS组相比，干预结束时FUL组创缘组织NF-κB、IL-1β、IL-6、TNF-α及MDA水平明显下降（

0.05），而SOD水平显著升高（

0.05），同时血清中IL-1β、IL-6、TNF-α表达明显下降（

0.05）。HE染色及Masson染色显示，与NS组相比，FUL组上皮结构连续、完整，胶原纤维更加密集且排列整齐，上皮爬行距离更远（

0.05），肉芽组织更厚（

0.05），胶原容积分数更高（

0.05）。

结论

富勒醇可以有效促进小鼠皮肤创面愈合，其可能是通过抑制NF-κB信号通路及下游炎症因子表达实现的。

Abstract

Objective

To explore the efficacy and mechanism of the biomaterial fullerenol （FUL） on skin wound healing of mice.

Methods

A total of 24 male C57BL/6 mice aged from 6 to 8 weeks were randomly divided into the sham operation group （control group）， model group+normal saline group （NS group） and the model group+fullerenol group （FUL group）， with 8 mice in each group.Mice in the FUL group injected intraperitoneally with 10 mg·kg

-1

·d

-1

fullerenol for 7 days，and mice in the NS group and the control group injected with an equal volume of normal saline.The daily body weight of mice were recorded， and the wounds were photographed on the 3rd and 7th days of treatment to calculate the corresponding wound healing rate.The wound skin tissue healing after treatment was observed by HE and Masson stainings， the mRNA levels of TNF-α，IL-6，IL-1β in wound margin tissue were detected by RT-qPCR，the protein levels of NF-κB，TNF-α，IL-6， IL-1β in wound margin tissue were detected by Western blot， the levels of TNF-α， IL-6， IL-1β in serum and MDA，SOD in wound margin tissue were detected by ELISA.

Results

There was no significant change in daily body weight among groups (

0.05).On the 3rd and 7th days， the wound healing rate of the FUL group was significantly higher than that of the NS group （

0.05）.Compared with the NS group， the levels of NF-κB， IL-1β， IL-6， TNF-α and MDA in the wound margin tissue of the FUL group were significantly decreased （

0.05）， while the level of SOD was significantly increased （

0.05）， and the expression of serum IL-1β， IL-6， and TNF-α were significantly decreased （

0.05）.The HE staining and Masson staining showed that the FUL group had a continuous and complete epithelial structure

denser and neatly arranged collagen fibers

longer epithelial crawl distance (

0.05)

thicker granulation tissue (

0.05)

and higher collagen volume fraction compared with the NS group (

0.05).

Conclusion

Fullerenol can effectively promote the skin wound healing of mice， which may be achieved by inhibiting the NF-κB signaling pathway and the expression of downstream inflammatory factors.

关键词

皮肤创面愈合富勒醇炎症NF-κB氧化应激

Keywords

skin woundhealingfullerenolinflammationNF-κBoxidative stress

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