甘草素对急性肾损伤小鼠的保护机制研究

胡佳丽; 曹卉; 雷茹; 崔娟; 崔婧

doi:10.11659/jjssx.07E022051

您当前的位置：

首页 >

文章列表页 >

甘草素对急性肾损伤小鼠的保护机制研究

基础研究 | 更新时间：2023-05-26

- 甘草素对急性肾损伤小鼠的保护机制研究
- Protective mechanism of liquiritigenin on acute kidney injury in mice
- 局解手术学杂志 2023年32卷第5期页码：389-393
- 作者机构：
  
  1.河北北方学院附属第一医院血液净化科，河北张家口 075000
  2.河北北方学院附属第一医院重症医学科，河北张家口 075000
- 作者简介：
  
  胡佳丽，E-mail：hujiali9361@163.com
- 基金信息：
  
  2021年河北省医学科学研究计划项目(20210759)
- DOI：10.11659/jjssx.07E022051
  中图分类号： R285.5
- 纸质出版日期：2023-05-25，
  
  收稿日期：2022-07-14，
扫描看全文
胡佳丽,曹卉,雷茹等.甘草素对急性肾损伤小鼠的保护机制研究[J].局解手术学杂志,2023,32(05):389-393.

HU Jia-li,CAO Hui,LEI Ru,et al.Protective mechanism of liquiritigenin on acute kidney injury in mice[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(05):389-393.
胡佳丽,曹卉,雷茹等.甘草素对急性肾损伤小鼠的保护机制研究[J].局解手术学杂志,2023,32(05):389-393. DOI： 10.11659/jjssx.07E022051.

HU Jia-li,CAO Hui,LEI Ru,et al.Protective mechanism of liquiritigenin on acute kidney injury in mice[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(05):389-393. DOI： 10.11659/jjssx.07E022051.

摘要

目的

探究甘草素对脂多糖（LPS）诱导的急性肾损伤小鼠的保护作用机制。

方法

将雄性昆明小鼠随机分为对照组、LPS组、LPS+甘草素3.75 mg/kg组、LPS+甘草素7.5 mg/kg组、LPS+甘草素15 mg/kg组和LPS+地塞米松组，每组8只。苏木素-伊红（HE）染色观察肾组织病理变化；全自动生化分析仪检测血清肌酐和血清尿素氮水平。酶联免疫吸附测定（ELISA）检测肾组织中IL-6、IL-1β和TNF-α水平；Western blot检测肾组织中IκBα和NF-κBp65蛋白的磷酸化水平和Toll样受体4（TLR4）、NOD样受体热蛋白结构域相关蛋白3(NLRP3)、Caspase-1、IL-1β蛋白表达水平。

结果

与对照组相比，LPS组小鼠肾组织中有大量炎症细胞浸润，血清肌酐、血清尿素氮、IL-6、IL-1β、TNF-α水平及p-IκBα/IκBα、p-NF-κBp65/NF-κBp65、TLR4、NLRP3、Caspase-1、IL-1β均显著上调，差异均有统计学意义（

0.05）。然而在LPS+甘草素7.5 mg/kg组、LPS+甘草素15 mg/kg组和LPS+地塞米松组中，这些指标变化均发生逆转。

结论

甘草素可能通过抑制NLRP3和NF-κB信号通路缓解LPS诱导的小鼠急性肾损伤。

Abstract

Objective

To explore the protective mechanism of liquiritigenin on lipopolysaccharide （LPS）-induced acute kidney injury in mice.

Methods

Male Kunming mice were randomly divided into the control group，the LPS group，the LPS+liquiritigenin 3.75 mg/kg group，the LPS+liquiritigenin 7.5 mg/kg group，the LPS+liquiritigenin 15 mg/kg group and the LPS+dexamethasone group，with 8 mice in each group.The pathologic changes of kidney tissue were observed by hematoxylin-eosin （HE） staining.The levels of serum creatinine and serum urea nitrogen were detected by automatic biochemical analyzer.The levels of IL-6，IL-1β and TNF-α in renal tissue were detected by enzyme-linked immunosorbent assay （ELISA）.Western blot was used to detect the phosphorylation levels of IκBα and NF-κBp65 protein and the expression levels of Toll-like receptor 4 （TLR4），NOD-like receptor thermal protein domain associated protein 3（NLRP3），Caspase-1 and IL-1β proteins in renal tissue.

Results

Compared with the control group，there were a large number of inflammatory cell infiltration in the renal tissue of mice in the LPS group，and the levels of serum creatinine，serum urea nitrogen，IL-6，IL-1β and TNF-α and p-IκBα/IκBα，p-NF-κBp65/NF-κBp65，TLR4，NLRP3，Caspase-1 and IL-1β were significantly up-regulated，the differences were statistically significant （

0.05）.However，the changes of these indexes were reversed in the LPS+liquiritigenin 7.5 mg/kg group，the LPS+liquiritigenin 15 mg/kg group and the LPS+dexamethasone group.

Conclusion

Liquiritigenin may alleviate LPS-induced acute kidney injury in mice by inhibiting NLRP3 and NF-κB signaling pathways.

关键词

甘草素脂多糖炎症NLRP3通路NF-κB通路

Keywords

liquiritigeninlipopolysaccharideinflammationNLRP3 pathwayNF-κB pathway

references

Manrique-Caballero CL，Del Rio-Pertuz G，Gomez H.Sepsis-associated acute kidney injury［J］.Crit Care Clin，2021，37（2）：279-301.doi：10.1016/j.ccc.2020.11.010http://dx.doi.org/10.1016/j.ccc.2020.11.010.

Fan H，Su BJ，Le JW，et al.Salidroside protects acute kidney injury in septic rats by inhibiting inflammation and apoptosis［J］.Drug Des Devel Ther，2022，16：899-907.doi：10.2147/DDDT.S361972http://dx.doi.org/10.2147/DDDT.S361972.

Li Y，Nourbakhsh N，Pham H，et al.Evolution of altered tubular

metabolism and mitochondrial function in sepsis-associated acute

kidney injury［J］.Am J Physiol Renal Physiol，2020，319（2）：F229-F244.doi：10.1152/ajprenal.00390.2019http://dx.doi.org/10.1152/ajprenal.00390.2019.

Wang Z，Wu J，Hu Z，et al.Dexmedetomidine alleviates lipopolysaccharide-induced acute kidney injury by inhibiting p75NTR-mediated oxidative stress and apoptosis［J］.Oxid Med Cell Longev，2020，2020：5454210.doi：10.1155/2020/5454210http://dx.doi.org/10.1155/2020/5454210.

Liu Z，Tang C，He L，et al.The negative feedback loop of NF-κB/miR-376b/NFKBIZ in septic acute kidney injury［J］.JCI Insight，2020，5（24）：e142272.doi：10.1172/jci.insight.142272http://dx.doi.org/10.1172/jci.insight.142272.

Kim YG，Kim SM，Kim KP，et al.The role of inflammasome-dependent and inflammasome-independent NLRP3 in the kidney［J］.Cells，2019，8（11）：1389-1401.doi：10.3390/cells8111389http://dx.doi.org/10.3390/cells8111389.

Shi Y，Wang H，Zheng M，et al.Ginsenoside Rg3 suppresses the NLRP3 inflammasome activation through inhibition of its assembly［J］.FASEB J，2020，34（1）：208-221.doi：10.1096/fj.201901537Rhttp://dx.doi.org/10.1096/fj.201901537R.

石盘棋，周博，孙晓燕，等.甘草黄酮苷元甘草素的研究及其应用［J］.解放军医药杂志，2020，32（5）：108-112.doi：10.3969/j.issn.2095-140X.2020.05.026http://dx.doi.org/10.3969/j.issn.2095-140X.2020.05.026.

Zhu X，Shi J，Li H.Liquiritigenin attenuates high glucose-induced

mesangial matrix accumulation，oxidativestress，and inflammation by suppression of the NF-κB and NLRP3 inflammasome pathways［J］.Biomed Pharmacother，2018，106：976-982.doi：10.1016/j.biopha. 2018.07.045http://dx.doi.org/10.1016/j.biopha.2018.07.045.

Gameiro J，Fonseca JA，Outerelo C，et al.Acute kidney injury：from diagnosis to prevention and treatment strategies［J］.J Clin Med，2020，9（6）：1704.doi：10.3390/jcm9061704http://dx.doi.org/10.3390/jcm9061704.

Gu H，Gwon MG，Kim JH，et al.Oridonin attenuates cisplatin-induced acute kidney injury via inhibiting oxidative stress，apoptosis，and

inflammation in mice［J］.Biomed Res Int，2022，2022：3002962.doi：10.1155/2022/3002962http://dx.doi.org/10.1155/2022/3002962.

Hongyan L，Suling W，Weina Z，et al.Antihyperuricemic effect of liquiritigenin in potassium oxonate-induced hyperuricemic rats［J］.Biomed Pharmacother，2016，84：1930-1936.doi：10.1016/j.biopha. 2016.11.009http://dx.doi.org/10.1016/j.biopha.2016.11.009.

Huang Z，Zhao Q，Chen M，et al.Liquiritigenin and liquiritin alleviated monocrotaline-induced hepatic sinusoidal obstruction syndrome via inhibiting HSP60-induced inflammatory injury［J］.Toxicology，2019，428：152307.doi：10.1016/j.tox.2019.152307http://dx.doi.org/10.1016/j.tox.2019.152307.

Shi C，Wu H，Xu K，et al.Liquiritigenin-loaded submicron emulsion protects against doxorubicin-induced cardiotoxicity via antioxidant，anti-inflammatory，and anti-apoptotic activity［J］.Int J Nanomedicine，2020，15：1101-1115.doi：10.2147/IJN.S235832http://dx.doi.org/10.2147/IJN.S235832.

Peerapornratana S，Manrique-Caballero CL，Gómez H，et al.Acute kidney injury from sepsis：current concepts，epidemiology，pathophy-siology，prevention and treatment［J］.Kidney Int，2019，96（5）：1083-1099.doi：10.1016/j.kint.2019.05.026http://dx.doi.org/10.1016/j.kint.2019.05.026.

Qi J，Rong Y，Wang L，et al.Rab7b overexpression-ameliorated

ischemic brain damage following tMCAO involves suppression of TLR4 and NF-κB p65［J］.J Mol Neuro Sci，2019，68（2）：163-170.doi：10.1007/s12031-019-01295-yhttp://dx.doi.org/10.1007/s12031-019-01295-y.

Li B，Liu C，Tang K，et al.Aquaporin-1 attenuates macrophage-mediated inflammatory responses by inhibiting p38 mitogen-activated protein kinase activation in lipopolysaccharide-induced acute kidney injury［J］.Inflamm Res，2019，68（12）：1035-1047.doi：10.1007/s00011-019-01285-1http://dx.doi.org/10.1007/s00011-019-01285-1.

Gao H，Kang N，Hu C，et al.Ginsenoside Rb1 exerts anti-inflammatory effects in vitro and in vivo by modulating toll-like receptor 4 dimerization and NF-κB/MAPKs signaling pathways［J］.Phytomedicine，2020，69：153197.doi：10.1016/j.phymed.2020.53197http://dx.doi.org/10.1016/j.phymed.2020.53197.

Chen M，Zhang C，Zhang J，et al.The involvement of DAMPs-mediated inflammation in cyclophosphamide-induced liver injury and the protection of liquiritigenin and liquiritin［J］.Eur J Pharmacol，2019，856：172421.doi：10.1016/j.ejphar.2019.172421http://dx.doi.org/10.1016/j.ejphar.2019.172421.

Zhang D，Ji P，Sun R，et al.Ginsenoside Rg1 attenuates LPS-induced chronic renal injury by inhibiting NOX4-NLRP3 signaling in mice［J］.Biomed Pharmacother，2022，150：112936.doi：10.1016/j.biopha.2022.112936http://dx.doi.org/10.1016/j.biopha.2022.112936.

Borges-Rodriguez M，Shields CA，Travis OK，et al.Platelet inhibition prevents NLRP3 inflammasome activation and sepsis-induced kidney injury［J］.Int J Mol Sci，2021，22（19）：10330.doi：10.3390/ijms221910330http://dx.doi.org/10.3390/ijms221910330.

Komada T，Muruve DA.The role of inflammasomes in kidney disease［J］.Nat Rev Nephrol，2019，15（8）：501-520.doi：10.1038/s41581-019-0158-zhttp://dx.doi.org/10.1038/s41581-019-0158-z.

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

金丝桃甙对百草枯诱导的大鼠急性肺损伤的保护作用

富勒醇促进小鼠皮肤创面愈合的疗效及机制

炎症相关信号通路在帕金森病中的研究进展

PAD4介导的瓜氨酸化组蛋白在中耳炎发病进程中的机制研究

氯胺酮联合电休克对抑郁样大鼠海马炎症及β淀粉样蛋白的影响