大鼠主动脉支架术模型的建立及光学相干断层扫描评估

邓梦杨; 张靖宇; 周登禄; 王贵学; 严文华; 赵晓辉

doi:10.11659/jjssx.05E023049

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大鼠主动脉支架术模型的建立及光学相干断层扫描评估

基础研究 | 更新时间：2023-10-23

- 大鼠主动脉支架术模型的建立及光学相干断层扫描评估
- Establishment of rat aortic stenting model and evaluation by optical coherence tomography
- 局解手术学杂志 2023年32卷第10期页码：850-853
- 作者机构：
  
  1.陆军军医大学新桥医院心内科，重庆 400037
  2.陆军军医大学学员基础医学院三大队，重庆 400038
  3.重庆大学生物工程学院，重庆 400044
  4.重庆医科大学附属第二医院血管疝腹壁外科，重庆 400010
- 作者简介：
  
  严文华，E-mail：306331@cqmu.edu.cn
  赵晓辉，E-mail：doctorzhaoxiaohui@yahoo.com
- 基金信息：
  
  中国博士后科学基金(2022MD713708);重庆市自然科学基金(cstc2021jcyj-bshX0069)
- DOI：10.11659/jjssx.05E023049
  中图分类号： R-33
- 纸质出版日期：2023-10-25，
  
  收稿日期：2023-05-10，
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邓梦杨,张靖宇,周登禄等.大鼠主动脉支架术模型的建立及光学相干断层扫描评估[J].局解手术学杂志,2023,32(10):850-853.

DENG Meng-yang,ZHANG Jing-yu,ZHOU Deng-lu,et al.Establishment of rat aortic stenting model and evaluation by optical coherence tomography[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(10):850-853.
邓梦杨,张靖宇,周登禄等.大鼠主动脉支架术模型的建立及光学相干断层扫描评估[J].局解手术学杂志,2023,32(10):850-853. DOI： 10.11659/jjssx.05E023049.

DENG Meng-yang,ZHANG Jing-yu,ZHOU Deng-lu,et al.Establishment of rat aortic stenting model and evaluation by optical coherence tomography[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(10):850-853. DOI： 10.11659/jjssx.05E023049.

摘要

目的

建立大鼠主动脉支架术动物模型，为冠状动脉支架再狭窄等研究提供实用的小动物平台。

方法

SD雄性大鼠18只(500~700 g)，麻醉消毒后，经左髂动脉切口于肾动脉分叉处上方10 mm的腹主动脉处行2.5 mm×16.0 mm支架植入，缝合左髂动脉切口。术前及术后即刻、1个月、2个月、6个月对支架段血管进行光学相干断层扫描(OCT)检测分析。

结果

通过切开髂动脉于大鼠腹主动脉行支架植入术均获成功，无大鼠死亡及跛行。OCT检查结果显示：术前大鼠主动脉内膜显示清晰，直径约2 mm；术后即刻支架钢梁突出内膜、贴壁良好；术后1个月可见内皮细胞覆盖；术后2个月见少量内膜增生；术后6个月可见较明显的内膜增生，未见血栓形成。

结论

大鼠经髂动脉切开主动脉支架植入可行性强，可通过OCT进行精准评估。

Abstract

Objective

An animal model of aortic stenting in rats was established to provide a practical small animal platform for the study of in-stent restenosis.

Methods

SD male rats （

=18，500~700 g） were anesthetized and disinfected. Drug-eluting stents （2.5 mm×16 mm） were implanted into the abdominal aorta 10 mm above the bifurcation of the renal artery via left iliac artery incision，and the left iliac artery incision was sutured. The vessel is evaluated by optical coherence tomography（OCT） before operation， immediately after operation，1 month，2 months and 6 months after operation.

Results

Stents were successfully implanted into the aorta of all rats via iliac artery incision， without death or lameness. The results of OCT showed that the intima of the aorta was clear before operation， with a diameter of about 2 mm； Immediately after operation， the stent steel beam had prominent intima and good adhesion； Endothelial cell covered the stent 1 month after operation； A small amount of intimal hyperplasia was observed 2 months after operation； There was obvious intimal hyperplasia 6 months after operation，and no thrombosis was found.

Conclusion

The stent implantation through iliac artery incision in aorta of rats is highly feasible and can be accurately evaluated by OCT.

关键词

大鼠主动脉支架光学相干断层扫描

Keywords

rataortastentingoptical coherence tomography

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