功能性纳米自组装多肽KLPP凝胶修复软骨缺损的实验研究

于鹏; 贾丙申; 段炼; 付昆

doi:10.11659/jjssx.04E023007

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功能性纳米自组装多肽KLPP凝胶修复软骨缺损的实验研究

基础研究 | 更新时间：2023-10-23

- 功能性纳米自组装多肽KLPP凝胶修复软骨缺损的实验研究
- Experimental study of functionalized self-assembling nanofiber peptide KLPP hydrogel in repairing cartilage defects
- 局解手术学杂志 2023年32卷第10期页码：854-858
- 作者机构：
  
  海南医学院第一附属医院关节外科，海南海口 570102
- 作者简介：
- 基金信息：
  
  海南省卫生健康行业科研项目(21A200024)
- DOI：10.11659/jjssx.04E023007
  中图分类号： R681.3
- 纸质出版日期：2023-10-25，
  
  收稿日期：2023-04-03，
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于鹏,贾丙申,段炼等.功能性纳米自组装多肽KLPP凝胶修复软骨缺损的实验研究[J].局解手术学杂志,2023,32(10):854-858.

.Experimental study of functionalized self-assembling nanofiber peptide KLPP hydrogel in repairing cartilage defects[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(10):854-858.
于鹏,贾丙申,段炼等.功能性纳米自组装多肽KLPP凝胶修复软骨缺损的实验研究[J].局解手术学杂志,2023,32(10):854-858. DOI： 10.11659/jjssx.04E023007.

.Experimental study of functionalized self-assembling nanofiber peptide KLPP hydrogel in repairing cartilage defects[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(10):854-858. DOI： 10.11659/jjssx.04E023007.

摘要

目的

探讨功能性纳米自组装多肽KLPP凝胶对关节软骨缺损的修复作用。

方法

培养人骨髓间充质干细胞（BMSCs），将BMSCs分为BMSCs-KLD-12组（BMSCs与KLD-12凝胶共培养）和BMSCs-KLPP组（BMSCs与KLPP凝胶共培养），分别行软骨诱导分化培养，MTT法检测细胞的增殖活性。将人软骨组织分为软骨-KLPP组（制作软骨缺损区并填充KLPP凝胶进行培养）、软骨-KLD-12组（制作软骨缺损区并填充KLD-12凝胶进行培养）、软骨组（仅制作软骨缺损区）。Calcein-AM/PI双染法检测BMSCs-KLD-12组及BMSCs-KLPP组的细胞毒性。培养49 d后，收集软骨组、软骨-KLD-12组、软骨-KLPP组的软骨组织进行切片，并行阿尔新蓝染色。Western blot法检测各组中Ⅰ型胶原（Col-Ⅰ）、Ⅱ型胶原（Col-Ⅱ）、Ⅹ型胶原（Col-Ⅹ）的表达。

结果

与BMSCs-KLD-12组比较，BMSCs-KLPP组培养1 d、3 d、7 d的细胞增殖率均明显增加（

0.05），且Col-Ⅰ、Col-Ⅱ、Col-Ⅹ的相对表达水平明显增加（

0.05）。Calcein-AM/PI双染色检测表明BMSCs-KLPP组和BMSCs-KLD-12组细胞活性基本一致，KLPP凝胶无细胞毒性。与软骨-KLD-12组比较，软骨-KLPP组软骨修复作用更明显（

0.05），且Col-Ⅰ、Col-Ⅱ、Col-Ⅹ的相对表达水平更高（

0.05）。

结论

功能性纳米自组装多肽KLPP凝胶对关节软骨缺损具有良好的修复作用。

Abstract

Objective

To investigate the repair effect of functionalized self-assembling nanofiber peptide KLPP hydrogel on articular cartilage defects.

Methods

Human bone marrow mesenchymal stem cells （BMSCs） were cultured and divided into the BMSCs-KLD-12 group （BMSCs co-cultured with KlD-12 hydrogel） and the BMSCs-KLPP group （BMSCs co-cultured with KLPP hydrogel）， and chondrogenic differentiation of cells were cultured， respectively. The proliferative activity of BMSCs was detected by MTT assay. Human cartilage tissues were divided into the chondro-KLPP group （cartilage defect area was prepared and cultured with KLPP hydrogel）， the chondro-KLD-12 group （cartilage defect area was prepared and cultured with KLD-12 hydrogel）， and the cartilage group （cartilage defect area was prepared only）. The cytotoxicity of the BMSCs-KLD-12 group and the BMSCS-KLPP group was detected by Calcein-AM/PI double staining method. After 49 days of culture， the cartilage tissues of the cartilage group， the chondro-KLD-12 group and the chondro-KLPP group were sliced and stained with Alcian blue. Western blot assay was used to detect the expression of type Ⅰ collagen （Col-Ⅰ）， type Ⅱ collagen （Col-Ⅱ） and type Ⅹ collagen （Col-Ⅹ） in all groups.

Results

Compared with the BMSCs-KLD-12 group， the proliferation rate of the BMSCs-KLPP group was significantly increased at day 1， day 3 and day 7 （

0.05）， and the relative expression levels of Col-Ⅰ， Col-Ⅱ and Col-Ⅹ were significantly increased （

0.05）. Calcein-AM/PI double staining showed that the cell activity of the BMSCs-KLPP group and the BMSCs-KLD-12 group was basically the same，and KLPP hydrogel had no cytotoxicity.Compared with the chondro-KLD-12 group，the cartilage repair effect of the chondro-KLPP group was significantly increased（

0.05）， and the relative expression levels of Col-Ⅰ， Col-Ⅱ and Col-Ⅹ were significantly increased （

0.05）.

Conclusion

Functional self-assembling nanofiber peptide KLPP hydrogel has a good repair effect on articular cartilage defects.

关键词

功能性纳米自组装多肽凝胶软骨细胞软骨组织缺损修复

Keywords

functionalized self-assembling nanofiber peptide hydrogelcartilage cellscartilage tissue defectsrepair

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