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TSP-1对小鼠皮肤放创复合伤伤口愈合的影响
Effect of TSP-1 on wound healing of skin combined radiation-wound injury in mice
- 局解手术学杂志 2023年32卷第10期 页码：865-869
- 作者机构：
陆军军医大学军事预防医学系复合伤研究所，重庆 400038
- 作者简介：
郝玉徽，E-mail：yuhui_hao@126.com
- 基金信息：国家自然科学基金(31024643)
DOI：10.11659/jjssx.03E023173
中图分类号： R146纸质出版日期：2023-10-25，
收稿日期：2023-03-28，
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南莎,柳随义,冉永红等.TSP-1对小鼠皮肤放创复合伤伤口愈合的影响[J].局解手术学杂志,2023,32(10):865-869.
NAN Sha,LIU Sui-yi,RAN Yong-hong,et al.Effect of TSP-1 on wound healing of skin combined radiation-wound injury in mice[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(10):865-869.
南莎,柳随义,冉永红等.TSP-1对小鼠皮肤放创复合伤伤口愈合的影响[J].局解手术学杂志,2023,32(10):865-869. DOI： 10.11659/jjssx.03E023173.
NAN Sha,LIU Sui-yi,RAN Yong-hong,et al.Effect of TSP-1 on wound healing of skin combined radiation-wound injury in mice[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2023,32(10):865-869. DOI： 10.11659/jjssx.03E023173.
摘要
目的
2
探讨TSP-1在小鼠皮肤放创复合伤（CRWI）伤口愈合过程中的作用。
方法
2
以TSP-1敲除（KO）小鼠为主要研究对象，以野生型（WT）小鼠作为对照，每组15只，比较WT小鼠与TSP-1KO小鼠皮肤CRWI的伤口愈合情况。取WT小鼠与TSP-1KO小鼠皮肤CRWI建模后第7天的伤口组织进行免疫组化染色以及免疫荧光染色，Western blot分析NF-кB蛋白表达水平，并使用RT-qPCR检测伤口部位炎症细胞因子IL-1β、IL-6和TNF-α的表达水平，以确定2组小鼠皮肤CRWI伤口部位的炎症反应状态及血管生成情况。
结果
2
与WT小鼠相比，从CRWI建模第3天开始TSP-1KO小鼠皮肤CRWI伤口愈合显著延迟（
P
<
0.05）。CRWI建模后第7天TSP-1KO小鼠伤口部位的F4/80巨噬细胞浸润减少（
P
<
0.05），NF-кB蛋白表达水平降低（
P
<
0.01），炎症细胞因子IL-1β和IL-6的表达水平也相对降低（
P
<
0.01），并且伤口部位CD31阳性细胞以及α-SMA阳性细胞数量减少（
P
<
0.05）。
结论
2
敲除TSP-1会导致小鼠皮肤CRWI炎症反应减弱，血管生成受到抑制，伤口愈合延迟。
Abstract
Objective
2
To investigate the effect of TSP-1 on wound healing process of skin combined radiation-wound injury （CRWI） in mice.
Methods
2
TSP-1 knockout （KO） mice were used as the main study subjects， and wild-type （WT） mice were used as the control， with 15 mice in each group. The wound healing of skin CRWI between WT mice with TSP-1KO mice was compared. The wound tissues of WT mice and TSP-1KO mice on the 7th day after skin CRWI modeling were taken for immunohistochemical staining and immunofluorescence staining， the NF-кB protein expression levels were analyzed by Western blot， and the expression levels of inflammatory cytokines IL-1β，IL-6 and TNF-α in the wound site were detected by RT-qPCR， so as to determine the inflammatory response and angiogenesis in the skin CRWI wound site of mice in the two groups.
Results
2
Compared with WT mice， skin CRWI wound healing was significantly delayed in TSP-1KO mice from the 3th day of CRWI modeling （
P
<
0.05）. On the 7th day after CRWI modeling， the infiltration of F4/80 macrophages in the wound site of TSP-1KO mice decreased （
P
<
0.05）， the NF-кB protein expression level decreased （
P
<
0.01）， the inflammatory cytokines IL-1β and IL-6 expression levels were also relatively reduced （
P
<
0.01）， and the number of CD31-positive cells as well as α-SMA-positive cells in the wound site were decreased （
P
<
0.05）.
Conclusion
2
Knockout of TSP-1 can weaken the inflammatory response， inhibit angiogenesis and delay wound healing of skin CRWI in mice.
关键词
TSP-1放创复合伤伤口愈合炎症反应血管生成
Keywords
TSP-1combined radiation-wound injurywound healinginflammatory responseangiogenesis
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