终丝牵拉脊髓拴系综合征动物模型的建立及评估

蒋擎玉; 尚爱加; 史旭东; 程浩峰; 苏天崎; 吴言

doi:10.11659/jjssx.02E024036

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终丝牵拉脊髓拴系综合征动物模型的建立及评估

基础研究 | 更新时间：2024-11-18

- 终丝牵拉脊髓拴系综合征动物模型的建立及评估
- Establishment and evaluation of animal model of filum terminale traction tethered cord syndrome
- 局解手术学杂志 2024年33卷第11期页码：985-990
- 作者机构：
  
  1.中国人民解放军医学院，北京 100853
  2.中国人民解放军总医院神经外科医学部，北京 100853
  3.南开大学医学院神经外科，天津 300071
- 作者简介：
  
  尚爱加，E-mail：Shangaj@126.com
- 基金信息：
  
  国家重点研发计划(2022YFC2703304);首都卫生发展科研专项(2022-2-5022)
- DOI：10.11659/jjssx.02E024036
  中图分类号： R744.9
- 纸质出版日期：2024-11-25，
  
  收稿日期：2024-02-19，
- 稿件说明：
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蒋擎玉,尚爱加,史旭东等.终丝牵拉脊髓拴系综合征动物模型的建立及评估[J].局解手术学杂志,2024,33(11):985-990.

JIANG Qing-yu,SHANG Ai-jia,SHI Xu-dong,et al.Establishment and evaluation of animal model of filum terminale traction tethered cord syndrome[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2024,33(11):985-990.
蒋擎玉,尚爱加,史旭东等.终丝牵拉脊髓拴系综合征动物模型的建立及评估[J].局解手术学杂志,2024,33(11):985-990. DOI： 10.11659/jjssx.02E024036.

JIANG Qing-yu,SHANG Ai-jia,SHI Xu-dong,et al.Establishment and evaluation of animal model of filum terminale traction tethered cord syndrome[J].JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY,2024,33(11):985-990. DOI： 10.11659/jjssx.02E024036.

摘要

目的

建立一种新的终丝牵拉脊髓拴系综合征动物模型，以探讨其发病机制。

方法

将16只新西兰大白兔随机分为牵拉组和假手术组，每组8只。牵拉组用丝线建立终丝牵拉脊髓拴系综合征模型，假手术组仅切断终丝但不作牵引。8周后，完成行为学Talov评分、腰骶部MRI检查、体感诱发电位检测、尿动力学指标检查及病理组织分析。

结果

术后第8周，牵拉组后肢损伤明显，术后第4周、8周Talov评分低于假手术组（

0.001）。术后8周腰骶部MRI结果显示，牵拉组矢状位MRI可见末端终丝受丝线牵引、膀胱异常膨大，轴位MRI可见椎管内脊髓受向下及向背侧方向的机械力；假手术组矢状位、轴位MRI显示，脊髓位于椎管正中，膀胱大小正常。术后第8周，牵拉组波幅显著低于假手术组（

0.001），波幅下降幅度超过50%；牵拉组总体潜伏期比假手术组略有延长（

0.05）。尿动力学检查结果显示，牵拉组的最大膀胱容量明显高于假手术组（

=-3.361，

0.001），膀胱压力明显低于假手术组（

=-3.361，

0.001），膀胱顺应性明显高于假手术组（

0.001）。病理学染色显示，终丝对脊髓的牵拉导致神经组织损伤、膀胱上皮细胞变性。

结论

本研究成功建立了新西兰大白兔终丝牵拉脊髓拴系综合征模型，可为探究脊髓拴系的发病机制和了解脊髓损伤的病理过程提供参考。

Abstract

Objective

To establish a new animal model of filum terminale traction tethered cord syndrome to explore its pathogenesis.

Methods

Sixteen New Zealand white rabbits were randomly divided into the traction group and the sham group， with 8 rabbits in each group. The traction group used silk thread to establish a model of filum terminale traction tethered cord syndrome， while the sham group only cut the filum terminale without traction. After 8 weeks， the behavioral Talov score， lumbosacral MRI examination， somatosensory evoked potential detection， urodynamic index test and pathological analysis were completed.

Results

At the 8th week after surgery， the hindlimb injury was obvious in the traction group， and the Talov scores at the 4th and 8th weeks after operation were lower than those in the sham group （

0.001）.The lumbosacral MRI results at 8 weeks after surgery showed that the distal filum terminale was pulled by silk thread，with bladder abnormal enlargement in sagital MRI in the traction group， while axial MRI showed the spinal cord within the spinal canal was subjected to mechanical forces in the downward and dorsal directions； the sagittal and axial MRI of the sham group showed that the spinal cord was located in the midd

le of the spinal canal and the bladder size was normal.At the 8th week after surgery， the amplitude in the traction group was significantly lower than that in the sham group （

0.001）， and the amplitude decreased by more than 50%. The overall latency period in the traction group was slightly longer than that in the sham group （

0.05）.The results of urodynamic examination showed that the maximum bladder capacity in the traction group was significantly higher than that in the sham group （

=-3.361，

0.001）， the bladder pressure was significantly lower than that in the sham group （

=-3.361，

0.001）， and the bladder compliance was significantly higher than that in the sham group （

0.001）.Pathological staining showed that the traction of the filum terminale on the spinal cord led to nerve tissue damage and degeneration of bladder epithelial cells.

Conclusion

This study successfully established a model of filum terminale traction tethered cord syndrome of New Zealand white rabbits， which can provide reference for exploring the pathogenesis of tethered cord and understanding the pathological process of spinal cord injury.

关键词

动物模型终丝脊髓拴系综合征病理染色尿动力学核磁共振体感诱发电位

Keywords

animal modelfilum terminaletethered cord syndromepathological stainingurodynamicsmagnetic resonance imagingsomatosensory evoked potential

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