<em>Zinc finger protein 831</em> promotes apoptosis and enhances chemosensitivity in breast cancer by acting as a novel transcriptional repressor targeting the <em>STAT3/Bcl2</em> signaling pathway

Jun Fan; Zhe Zhang; Hongqiang Chen; Dongjiao Chen; Wenbo Yuan; Jingzhi Li; Yong Zeng; Shimeng Zhou; Shu Zhang; Gang Zhang; Jiashen Xiong; Lu Zhou; Jing Xu; Wenbin Liu; Yan Xu

doi:10.1016/j.gendis.2022.11.023

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Zinc finger protein 831 promotes apoptosis and enhances chemosensitivity in breast cancer by acting as a novel transcriptional repressor targeting the STAT3/Bcl2 signaling pathway

Full Length Articles | 更新时间：2024-02-04

- Zinc finger protein 831 promotes apoptosis and enhances chemosensitivity in breast cancer by acting as a novel transcriptional repressor targeting the STAT3/Bcl2 signaling pathway
- Zinc finger protein 831 promotes apoptosis and enhances chemosensitivity in breast cancer by acting as a novel transcriptional repressor targeting the STAT3/Bcl2 signaling pathway
- Genes & Diseases 2024年1卷第1期页码：430-448
- 作者机构：
  
  1. Department of Breast and Thyroid Surgery, Daping Hospital, Army Medical University (Third Military Medical University),Chongqing,China,400042
  2. Institute of Toxicology, College of Preventive Medicine, Army Medical University (Third Military Medical University),Chongqing,China,400038
  3. Department of Environmental Health, College of Preventive Medicine, Army Medical University (Third Military Medical University),Chongqing,China,400038
  4. Anesthesia and Intensive Care, Chinese University of Hong Kong,Hong Kong SAR,China,999077
  5. School of Public Health, Xinxiang Medical University,Henan,Xinxiang,China,453003
  6. School of Public Health, China Medical University,Liaoning,Shenyang,China,110122
- 作者简介：
- 基金信息：
- DOI：10.1016/j.gendis.2022.11.023
  中图分类号：
- 纸质出版日期：2024，
  
  网络出版日期：2024-1-1，
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Zinc finger protein 831 promotes apoptosis and enhances chemosensitivity in breast cancer by acting as a novel transcriptional repressor targeting the STAT3/Bcl2 signaling pathway[J]. Genes & Diseases, 2024,1(1):430-448.

Jun Fan, Zhe Zhang, Hongqiang Chen, et al. Zinc finger protein 831 promotes apoptosis and enhances chemosensitivity in breast cancer by acting as a novel transcriptional repressor targeting the STAT3/Bcl2 signaling pathway[J]. Genes & Diseases, 2024,1(1):430-448.
Zinc finger protein 831 promotes apoptosis and enhances chemosensitivity in breast cancer by acting as a novel transcriptional repressor targeting the STAT3/Bcl2 signaling pathway[J]. Genes & Diseases, 2024,1(1):430-448. DOI： 10.1016/j.gendis.2022.11.023.

Jun Fan, Zhe Zhang, Hongqiang Chen, et al. Zinc finger protein 831 promotes apoptosis and enhances chemosensitivity in breast cancer by acting as a novel transcriptional repressor targeting the STAT3/Bcl2 signaling pathway[J]. Genes & Diseases, 2024,1(1):430-448. DOI： 10.1016/j.gendis.2022.11.023.

摘要

Abstract

Emerging evidence suggested that

zinc finger protein 831

(

ZNF831

) was associated with immune activity and stem cell regulation in breast cancer. Whereas

the roles and molecular mechanisms of

ZNF831

in oncogenesis remain unclear.

ZNF831

expression was significantly diminished in breast cancer which was associated with promoter CpG methylation but not mutation. Ectopic over-expression of

ZNF831

suppressed breast cancer cell proliferation and colony formation and promoted apoptosis

in vitro

while knockdown of

ZNF831

resulted in an opposite phenotype. Anti-proliferation effect of

ZNF831

was verified

in vivo

. Bioinformatic analysis of public databases and transcriptome sequencing both showed that

ZNF831

could enhance apoptosis through transcriptional regulation of the

JAK

STAT

pathway. ChIP and luciferase report assays demonstrated that ZNF831 could directly bind to one specific region of

STAT3

promoter and induce the transcriptional inhibition of

STAT3

. As a result

the attenuation of

STAT3

led to a restraint of the transcription of

Bcl2

and thus accelerated the apoptotic progression. Augmentation of

STAT3

diminished the apoptosis-promoting effect of

ZNF831

in breast cancer cell lines. Furthermore

ZNF831

could ameliorate the anti-proliferation effect of capecitabine and gemcitabine in breast cancer cell lines. Our findings demonstrate for the first time that

ZNF831

is a novel transcriptional suppressor through inhibiting the expression of

STAT3

Bcl

2 and promoting the apoptosis process in breast cancer

suggesting

ZNF831

as a novel biomarker and potential therapeutic target for breast cancer patients.

关键词

Keywords

ApoptosisBreast cancerChemosensitivitySTAT3ZNF831

references

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Zinc finger protein 831 promotes apoptosis and enhances chemosensitivity in breast cancer by acting as a novel transcriptional repressor targeting the STAT3/Bcl2 signaling pathway

Zinc finger protein 831 promotes apoptosis and enhances chemosensitivity in breast cancer by acting as a novel transcriptional repressor targeting the STAT3/Bcl2 signaling pathway

DOI：10.1016/j.gendis.2022.11.023

摘要

Abstract

关键词

Keywords

references