Dynamic human retinal pigment epithelium (RPE) and choroid architecture based on single-cell transcriptomic landscape analysis

Lulin Huang; Lin Ye; Runze Li; Shanshan Zhang; Chao Qu; Shujin Li; Jie Li; Mu Yang; Biao Wu; Ran Chen; Guo Huang; Bo Gong; Zheng Li; Hongjie Yang; Man Yu; Yi Shi; Changguan Wang; Wei Chen; Zhenglin Yang

doi:10.1016/j.gendis.2022.11.007

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Dynamic human retinal pigment epithelium (RPE) and choroid architecture based on single-cell transcriptomic landscape analysis

Full Length Articles | 更新时间：2024-02-04

- Dynamic human retinal pigment epithelium (RPE) and choroid architecture based on single-cell transcriptomic landscape analysis
- Dynamic human retinal pigment epithelium (RPE) and choroid architecture based on single-cell transcriptomic landscape analysis
- Genes & Diseases 2023年1卷第6期页码：2540-2556
- 作者机构：
  
  1. Sichuan Provincial Key Laboratory for Human Disease Gene Study, Center for Medical Genetics, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China,Sichuan,Chengdu,China,610072
  2. Research Unit for Blindness Prevention of Chinese Academy of Medical Sciences (2019RU026), Sichuan Academy of Medical Sciences,Sichuan,Chengdu,China,610072
  3. Department of Ophthalmology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China,Sichuan,Chengdu,China,610072
  4. School of Ophthalmology and Optometry, Wenzhou Medical College,Zhejiang,Wenzhou,China,325035
  5. Department of Organ Transplant Center, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China,Sichuan,Chengdu,China,610072
  6. Department of Ophthalmology, Peking University Third Hospital,Beijing,China,100730
- 作者简介：
- 基金信息：
- DOI：10.1016/j.gendis.2022.11.007
  中图分类号：
- 纸质出版日期：2023，
  
  网络出版日期：2023-11-1，
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Dynamic human retinal pigment epithelium (RPE) and choroid architecture based on single-cell transcriptomic landscape analysis[J]. Genes & Diseases, 2023,1(6):2540-2556.

Lulin Huang, Lin Ye, Runze Li, et al. Dynamic human retinal pigment epithelium (RPE) and choroid architecture based on single-cell transcriptomic landscape analysis[J]. Genes & Diseases, 2023,1(6):2540-2556.
Dynamic human retinal pigment epithelium (RPE) and choroid architecture based on single-cell transcriptomic landscape analysis[J]. Genes & Diseases, 2023,1(6):2540-2556. DOI： 10.1016/j.gendis.2022.11.007.

Lulin Huang, Lin Ye, Runze Li, et al. Dynamic human retinal pigment epithelium (RPE) and choroid architecture based on single-cell transcriptomic landscape analysis[J]. Genes & Diseases, 2023,1(6):2540-2556. DOI： 10.1016/j.gendis.2022.11.007.

摘要

Abstract

The retinal pigment epithelium (RPE) and choroid are located behind the human retina and have multiple functions in the human visual system. Knowledge of the RPE and choroid cells and their gene expression profiles are fundamental for understanding retinal disease mechanisms and therapeutic strategies. Here

we sequenced the RNA of about 0.3 million single cells from human RPE and choroids across two regions and seven ages

revealing regional and age differences within the human RPE and choroid. Cell–cell interactions highlight the broad connectivity networks between the RPE and different choroid cell types. Moreover

the transcription factors and their target genes change during aging. The coding of somatic variations increases during aging in the human RPE and choroid at the single-cell level. Moreover

we identified

ELN

as a candidate for improving RPE degeneration and choroidal structure during aging. The mapping of the molecular architecture of the human RPE and choroid improves our understanding of the human vision support system and offers potential insights into the intervention targets for retinal diseases.

关键词

Keywords

AgingChoroidELNHumanRPEscRNA-seq

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