A novel <em>in vitro</em> prognostic model of bladder cancer based on urine-derived living tumor cells

Jiaqi Wang; Jiying Zhu; Junchi Hu; Ziruoyu Wang; Xiaobo Wang; Jianbo Pan; Yiwei Chu; Zengxia Li; Wei Jiang; Chunmin Liang; Jun Hou; Jianming Guo; Yongjun Dang; Shuai Jiang

doi:10.1016/j.gendis.2022.10.022

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A novel in vitro prognostic model of bladder cancer based on urine-derived living tumor cells

Full Length Articles | 更新时间：2024-02-04

- A novel in vitro prognostic model of bladder cancer based on urine-derived living tumor cells
- A novel in vitro prognostic model of bladder cancer based on urine-derived living tumor cells
- Genes & Diseases 2023年1卷第6期页码：2586-2596
- 作者机构：
  
  1. Key Laboratory of Metabolism and Molecular Medicine, Ministry of Education and Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University,Shanghai,China,200032
  2. Department of Immunology, School of Basic Medical Sciences, Fudan University,Shanghai,China,200032
  3. Laboratory of Tumor Immunology, Department of Anatomy, Histology, and Embryology, School of Basic Medical Sciences, Fudan University,Shanghai,China,200032
  4. Center for Novel Target and Therapeutic Intervention, Chongqing Medical University,Chongqing,China,400016
  5. Department of Pathology, Zhongshan Hospital, Fudan University,Shanghai,China,200032
  6. Department of Urology, Zhongshan Hospital, Fudan University,Shanghai,China,200032
  7. Department of Urology, Zhongshan Hospital Wusong Branch, Fudan University,Shanghai,China,200940
- 作者简介：
- 基金信息：
- DOI：10.1016/j.gendis.2022.10.022
  中图分类号：
- 纸质出版日期：2023，
  
  网络出版日期：2023-11-1，
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A novel in vitro prognostic model of bladder cancer based on urine-derived living tumor cells[J]. Genes & Diseases, 2023,1(6):2586-2596.

Jiaqi Wang, Jiying Zhu, Junchi Hu, et al. A novel in vitro prognostic model of bladder cancer based on urine-derived living tumor cells[J]. Genes & Diseases, 2023,1(6):2586-2596.
A novel in vitro prognostic model of bladder cancer based on urine-derived living tumor cells[J]. Genes & Diseases, 2023,1(6):2586-2596. DOI： 10.1016/j.gendis.2022.10.022.

Jiaqi Wang, Jiying Zhu, Junchi Hu, et al. A novel in vitro prognostic model of bladder cancer based on urine-derived living tumor cells[J]. Genes & Diseases, 2023,1(6):2586-2596. DOI： 10.1016/j.gendis.2022.10.022.

摘要

Abstract

Bladder cancer (BLCA) remains a difficult malignancy to manage because of its high recurrence

intense follow-up

and invasive diagnostic and treatment techniques. Immune checkpoint inhibitors (ICIs) have forged a new direction for the treatment of BLCA

but it is currently challenging to predict whether an individual patient will be sensitive to ICIs. We collected 43 urine/tumor samples from BLCA patients for primary bladder cancer cells (BCCs) culturing using our previously reported BCC culture platform. We used flow cytometry (FCM) to measure the expression levels of Programmed Death-Ligand 1 (PD-L1) on BCCs before and after interferon-gamma (IFN-γ) treatment and found that PD-L1 expression and the sensitivities to IFN-γ varied among patients. RNA-sequencing

western blotting

and programmed death-1 (PD-1) binding assays confirmed that the BCC FCM-based PD-L1 detection platform (BC-PD-L1) was reliable and was not hindered by the glycosylation of PD-L1. In the subsequent retrospective study

we found that IFN-γ-stimulated PD-L1 (sPD-L1) expression on BCCs detected by BC-PD-L1 could predict the prognosis of BLCA patients. Importantly

the prognostic value was similar or even better in urine-derived BC-PD-L1 (UBC-PD-L1). Transcriptome analysis showed that BCCs with high sPD-L1 tended to enrich genes associated with the collagen-containing extracellular matrix

cell–cell adhesion

and positive regulation of the immune system. In addition

the UBC-PD-L1 also exhibited predictive value for ICI response in BLCA patients. In conclusion

as a novel personalized urine-detection method

UBC-PD-L1 may provide a rapid

accurate

and non-invasive tool for monitoring tumor progression

predicting therapeutic responses

and helping improve BLCA clinical treatment in future.

关键词

Keywords

Bladder cancerImmune checkpoint inhibitorsPredictive modelPrimary cellsUrine

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A novel in vitro prognostic model of bladder cancer based on urine-derived living tumor cells

A novel in vitro prognostic model of bladder cancer based on urine-derived living tumor cells

DOI：10.1016/j.gendis.2022.10.022

摘要

Abstract

关键词

Keywords

references