Objective　To explore teaching effectsof biochemistry by comparingthe traditional teaching with the network investigative teaching. Methods　425 five-year-program students of clinical medicine of grade 2012 were chosen as subjects， with 246 students from Classes 5-8 as experimental group in which network investigative teaching was implemented andwith 179 students of Classes 17-20 as the control group which wasgiven traditional teaching. After the teaching， the teaching effects were evaluated through a questionnaire and the test scores of the two groups were compared and statistically analyzed. The two groups were compared by using t test. Results　More than 70% of students in the experimental groups were generally satisfied with several aspects of the network investigative teaching and gavea good evaluation. The experimental group students' total scores and every item scores were all higher than the control group students'， in which the practise scores and the case analysis problem scores had significant differences by statistical analysis with P values being 0.000 and 0.002， respec-tively. Conclusion　Network investigative teaching is better than traditional teaching in the teaching of biochemistry， which can enhance students' ability of problem analysis and their enthusiasm for learning.