Objective　To explore the application value of comparative imaging combined with multimedia teaching in the five-year undergraduate internship teaching of nuclear medicine. Methods 70 undergraduates internship in the nuclear medicine department of our hospital from June 2017 to February 2018 were divided into the control group and experimental group

35 in each group. The control group adopted traditional teaching method

and the experimental group adopted comparative imaging combined with multimedia teaching for internship teaching. The theoretical test scores

the evaluation of the self-perception of the teaching process and the teaching feedback of the two groups were compared. Results The theoretical test scores of the experimental group students were significantly higher than those of the control group

including the noun explanation [(26.60±0.51) vs. (22.20±0.58)]

the short-answer question [(26.80±0.37) vs. (22.40±0.60)]

the essay question [(36.00±0.71) vs. (27.40±1.03)] and total score [(92.40±0.93) vs. (71.80±0.37)]. The satisfactions with the evaluation of the self-perception in the teaching process and the teaching feedback in the experimental group were also much better than those of the control group. Conclusion　The application of comparative imaging combined with multimedia teaching in the practice teaching for undergraduate students in nuclear medicine could effectively improve the clinical practice teaching quality and actively promote the development of the teaching of nuclear medicine