Objective　To investigate the teaching effect of interactive online teaching model in nursing teaching. Methods　Five classes （260 undergraduates of 2011 grade） in Hubei University of Medicine were randomly divided into experimental group and control group， 130 undergraduates in each group. Web-based instructions were carried out in control group and video interactive communi-cation tools were performed as auxiliary teaching in experiment group. The application effect was as-sessed by questionnaire survey. The overall satisfaction survey results were statistically analyzed by Chi-Square test and exam scores were statistically analyzed by two sample t-test. P<0.05 signifies sta-tistically significant difference. Results　The acceptance of teaching effectiveness in experimental group was 93.08%（121/130）， which was higher than that of 63.08% （82/130） in experimental group （ χ2=10.14， P=0.000）. Vivid of teaching， synchronous guiding， teaching resources sharing， real-time answering ability and attention of student， memorization of teaching content in experimental group were all better than those in control group; the score of two groups were respectively （89.47 ± 5.61） and （85.25 ± 5.49）， with statistical differences （t=3.520， P=0.000）. Conclusions　The interactive video network teaching mode is lively， interesting and mnemonic and has the functions of synchronous guiding， real-time answering， teaching resource sharing， which can improve scores of student.