Objective　Biochemistry course， a professional basic course of medical students， is a challenging course， for which the traditional classroom teaching mode is not suitable， for there are many deficiencies in traditional classroom teaching， so new teaching methods need to be explored to improve the quality of teaching and learning efficiency. Method　301 students from 6 natural classes of adult medical education were selected as the object of study and were divided into the experimental group（150） and control group（151）. The traditional teaching mode was adopted in the control group， while in the experimental group， the “double-track” teaching methods were adopted such as diagram method， the method of using body language， “humor” teaching method， to stimulate students' interest in scientific research and innovation. At the end of the course， students were asked to conduct online teaching evaluation while teachers were asked to conduct mutual evaluation. Examination paper analy-sis software was used to evaluate the examination paper. Test results were analyzed by using SPSS 15 Statistical Software for data processing. Results： mean ± standard deviation（x±s）； comparison was made between groups in t test. The difference was statistically significant（P<0.05）. Result　The eval-uation results of students and colleagues assessment show that 93.7% of the students and 90.9% of colleagues think that the experimental group uses the double-track teaching method is more popular than in the control group. At the end of the experiment， the control group grades is （66±25）， while the experimental group grades is （83±22）； t value is 6.2612； P value is 0.000. Conclusion　Com-pared with traditional teaching method double-track teaching method has obvious advantages in teaching. From the result， “double-track teaching” enables students willing to think and have an active participation and exploration， which has significantly stimulated their learning interest and improved their performance， thus achieving good results.