Zeng Chengmin, et al. ANALYSIS OF MAJOR PHOSPHOLIPID CLASSES IN BIOMEMBRANE BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY[J]. Journal of Chongqing Medical University, 1994,(2).
Zeng Chengmin, et al. ANALYSIS OF MAJOR PHOSPHOLIPID CLASSES IN BIOMEMBRANE BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY[J]. Journal of Chongqing Medical University, 1994,(2).DOI:
novel high performance liquid chromatographic method for analysis of phospholipids inbiomembrane is deseribed. The separation was performed on a CLC-SIL column with the mobilephase system of acetonitril-methanol-85%phosphoric acid
using isocratic elution and UVdetection at 205nm. completed separation of eight constituente of membrane phospholipids wasachieved within 45 min
and four major constituents of phospholipids in erythrocyte ghostmembrane could be separated completely within 12 min.This method is rapid
reliable
producibleand practical for determination of biomembrane phospholipids.