荧光探针Fura-2/AM测定大鼠肝细胞胞内游离钙离子浓度

徐勇; 杨清; 涂植光

您当前的位置：

首页 >

文章列表页 >

荧光探针Fura-2/AM测定大鼠肝细胞胞内游离钙离子浓度

更新时间：2024-01-17

- 荧光探针Fura-2/AM测定大鼠肝细胞胞内游离钙离子浓度
- Measurement of cytosolic Ca~2+ level of the isolated rat hepatocytes with fluorescent probe-fura - 2/AM
- 重庆医科大学学报 1997年第4期
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  中图分类号： R322.47
- 纸质出版日期：1997，
扫描看全文
[1]徐勇,杨清,涂植光.荧光探针Fura-2/AM测定大鼠肝细胞胞内游离钙离子浓度[J].重庆医科大学学报,1997(04):305-307.

Measurement of cytosolic Ca~2+ level of the isolated rat hepatocytes with fluorescent probe-fura - 2/AM[J]. Journal of Chongqing Medical University, 1997,(4).
[1]徐勇,杨清,涂植光.荧光探针Fura-2/AM测定大鼠肝细胞胞内游离钙离子浓度[J].重庆医科大学学报,1997(04):305-307. DOI：

Measurement of cytosolic Ca~2+ level of the isolated rat hepatocytes with fluorescent probe-fura - 2/AM[J]. Journal of Chongqing Medical University, 1997,(4). DOI：

摘要

采用胶原酶原位灌注法分离纯化了大鼠肝细胞，并用荧光探针Ｆｕｒａ－２／ＡＭ测定了细胞内游离钙离子浓度。结果显示，胶原酶原位灌注法可分离获得高纯度，高活率的单个大鼠肝细胞，适合用荧光探针Ｆｕｒａ－２／ＡＭ测定胞内游离钙离子浓度；铡得新分离大鼠肝细胞静息状态下细胞内游离钙离子浓度为１７４．５±５７．３ｎｍｏｌ／Ｌ(我们就上述两种技术进行了一定的改进和探讨。

Abstract

The rat hepatocytes were isolated in situ with collagenase perfusion method and the cytosolic Ca~2+ levels were measured using fluorescent probe fura - 2/AM . Some improvement on the two reported methods was made. The results suggested that isolated hepatocytes were of high purity and viability

and suitable for the measurement of cytosolic Ca~2+ with fura - 2/AM. The cytosolic Ca~2+ level of isolated rat hepatocytes in metabolic quiescent stage was:174.5± 57.3 nmol/L ( s)

关键词

肝细胞钙离子荧光光度法胶原酶类灌注法

Keywords

references

浏览量

370

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

暂无数据