人外周血树突状细胞的体外培养扩增

李用国; 罗云萍; 梁增伟; 任红

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人外周血树突状细胞的体外培养扩增

更新时间：2024-01-17

- 人外周血树突状细胞的体外培养扩增
- Proliferation of human dendritic cells from monocyte in vitro
- 重庆医科大学学报 2001年第1期页码：1-3
- 作者机构：
  
  重庆医科大学病毒性肝炎研究所,重庆,400010
- 作者简介：
- 基金信息：
  
  国家自然科学基金资助!（39970673）
- DOI：
  中图分类号： R392.12
- 纸质出版日期：2001，
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[1]李用国,罗云萍,梁增伟,任红.人外周血树突状细胞的体外培养扩增[J].重庆医科大学学报,2001(01):1-3.

Proliferation of human dendritic cells from monocyte in vitro[J]. Journal of Chongqing Medical University, 2001,(1):1-3.
[1]李用国,罗云萍,梁增伟,任红.人外周血树突状细胞的体外培养扩增[J].重庆医科大学学报,2001(01):1-3. DOI：

Proliferation of human dendritic cells from monocyte in vitro[J]. Journal of Chongqing Medical University, 2001,(1):1-3. DOI：

摘要

目的：在体外从人外周血中培养扩增树突状细胞。方法：从正常人外周血分离PBMC，经两次孵育粘附，弃去悬浮细胞后加细胞因子（GM-CSF，IL-4）和培养液进行培养，并对培养过程进行观察鉴定。结果：培养1周后即可培养出典型的树突状细胞，经FITC-CD1a单抗标记后用流式细胞仪检测DC的CD1a表达率为 65%，并能刺激同种淋巴细胞增殖反应。结论：利用GM-CSF和 IL-4可以从人外周血中扩增出大量DC，可为后续研究做准备。

Abstract

Objective: To proliferate dendritic cells from human peripheral blood. Methods: PBMC isolated from healthy human peripheral blood were incubated for 3 hours twice at 37℃ in humidified 5%CO2. After 3 hours

nonadherent cells were gently removed

then the adherent cells were cultured at 37℃ in humidified 5%CO2 in air

in medium with GM-CSF and IL-4 for more than 7 days. Finally

DCs were collected and identified in morphology，function and specific symbol of cell surface. Results: After 7 days

typical dendritic cells appeared and gradually became larger and more typical.By using flow cytometric cell surface marker analysis

the level of expressing CD1a was 65%. DCs could also induce proliferation of allogeneic lymphocytes. Conclusion: A large amount of DCs could be induced from peripheral blood monocyte by adding GM-CSF and IL-4.

关键词

树突状细胞单核细胞粒细胞-巨噬细胞集落刺激因子白细胞介素-4

Keywords

dendritic cellsmonocytehGM-CSFhIL-4

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