Objective: We observed the changes of myocardiocytes apoptosis and the expression of bcl-2mRNA after the intervention by myocardial ischemic preconditioning(IPC)

brief occlusion of anterior mesenteric artery (MAO) and femoral artery(FAO)

in order to obtain the partial cardial myoctes protective mechanism of them. Methods: 56 Wistar rats divided into I/R.

IPC

FAO

and MAO groups were tested

using in situ TdT-mediated dUTP nick end labeling(TUNEL)

and RT-PCR methods. Results: We obtained these ischemic preconditionings which reduced the rats` infarction size(35.9%±2.1%vs17.6%±1.7%

24.3%±1.7%

23.5±1.7%) by reducing the myocardiocyts apoptosis(24.67%±4.18vs9.56%±2.43%

13%±4.29%

15%±5.09%) through the upregulation of bcl-2 mRNA(0.82±0.02VS0.91±0.04

0.89±0.04

0.88±0.02). Conclusion: The trial results can be summarized as follows: IPC

FAO

and MAO can reduce the rats’ ventricular myocardial infarction size; the partial myocardial protective mechanisms of IPC

FAO

and MAO can be obtained by the upregulation of bcl-2 mRNA. The preventive effect of bax mRNA by these interventions is under research.