环磷酰胺对睾丸组织中SCF表达的影响及意义

代江涛; 李旭良; 魏光辉; 林涛; 何大维; 刘俊宏; 李禄生

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环磷酰胺对睾丸组织中SCF表达的影响及意义

更新时间：2024-01-17

- 环磷酰胺对睾丸组织中SCF表达的影响及意义
- Expression of stem cell factor of testes was effected by cytoxan
- 重庆医科大学学报 2006年第3期页码：334-337
- 作者机构：
  
  1. 重庆医科大学儿童医院泌尿外科
  2. 重庆医科大学儿童医院泌尿外科,重庆,400014
- 作者简介：
- 基金信息：
- DOI：
  中图分类号： R698.2
- 纸质出版日期：2006，
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[1]代江涛,李旭良,魏光辉,林涛,何大维,刘俊宏,李禄生.环磷酰胺对睾丸组织中SCF表达的影响及意义[J].重庆医科大学学报,2006(03):334-337.

DAI Jiangtao, et al. Expression of stem cell factor of testes was effected by cytoxan[J]. Journal of Chongqing Medical University, 2006,(3):334-337.
[1]代江涛,李旭良,魏光辉,林涛,何大维,刘俊宏,李禄生.环磷酰胺对睾丸组织中SCF表达的影响及意义[J].重庆医科大学学报,2006(03):334-337. DOI：

DAI Jiangtao, et al. Expression of stem cell factor of testes was effected by cytoxan[J]. Journal of Chongqing Medical University, 2006,(3):334-337. DOI：

摘要

目的:通过环磷酰胺对大鼠睾丸组织中干细胞因子的表达及增殖指数影响的研究

以探讨环磷酰胺所致生精功能损害的原因。方法:选取9天龄雄性Wistar大鼠

腹腔注射大、中、小剂量的环磷酰胺后24h、4w、8w分别采集睾丸标本

应用RT-PCR技术检测睾丸组织中SCF的表达

同时光镜下计数4w时睾丸组织的增殖指数。结果:（1）对照组随年龄的增加

睾丸组织中分泌型干细胞因子表达无显著差异（P>0.05）

膜型干细胞因子均有显著增加（P<0.05）。（2）同一时期各实验组和对照组比较

分泌型干细胞因子表达无显著差异（P>0.05）

但膜型干细胞因子表达均有显著降低（P<0.05）;同一时期各实验组间比较

随环磷酰胺剂量增加

膜型干细胞因子表达有显著降低（P<0.05）。（3）同一剂量三个时期比较

各实验组分泌型干细胞因子无显著差异（P>0.05）;膜型干细胞因子比较

大剂量组中无显著差异（P>0.05）

中、小剂量组中24h较4w、8w均有显著降低（P<0.05）

4w与8w比较无显著差异（P>0.05）。（4）增殖指数检测

4w时各实验组与对照组比较

均有显著降低（P<0.01）

并与剂量负相关。结论:本研究结果提示由于环磷酰胺的毒性作用

导致了睾丸组织中膜型干细胞因子表达下调

进而影响增殖指数降低

这可能即是临床上环磷酰胺使用后产生不同程度生精功能障碍的原因之一。

Abstract

Objective: To assess the effect of cytoxan results on testes by the expression of stem cell factor(SCF) and repopulation index of testes in rats.Methods: 9d male Wistar rats were injected intraperitoneally with cytoxan of large

medium

small doses.After 24h

SCF was detected by RT-PCR and repopulation index in 4w was counted by microscop of testes in rats.Results:(1) With increase of age

soluble SCF(sSCF)of control groups was not significantly different(P>0.05)

but transmembrane SCF(mSCF) of control groups were significantly increased(P<0.05).(2) Expression of SCF of experimental groups and control groups were compared with at the same period

sSCF were not significantly different(P>0.05)

but mSCF decreased significantly(P<0.05)

as in each experimental groups.(3) SCF were compared with same dose at three periods in experimental groups

sSCF were not significantly different(P>0.05)

mSCF of large doses groups were not significantly different(P>0.05)

mSCF of medium and small doses group in 24h were significantly lower than that of 4w and 8w

that of 4w and that of 8w was not significantly different(P>0.05).(4) Repopulation index of experimental groups in 4w were significantly lower than that of control groups(P<0.05)

meanwhile

it was negatively correlated with doses.Conclusions: The toxicity of cytoxan can reduce the expression of mSCF of testes in Wistar rat

which resulted in decrease of repopulation index

it maybe one of the effect that cytoxan injuried seminiferous function.

关键词

环磷酰胺干细胞因子增殖指数生精功能

Keywords

cytoxanStem cell factorRepopulation indexSeminiferous function

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