Objective: In the present study

We developed and assess an integrin-targeted fiber-mutant Ad vector containing the Arg-Gly-Asp(RGD) peptide motif on the fiber knob for the transfection of melanoma cell lines.Methods: We constructed RGD-Adv by in vitro ligation method

and investigated the efficiency of gene transdution in melanoma cells

studied its stability

in vitro

and in vivo toxicology.Results: For in vivo and in vitro

gene transfer the RGD-Adv more efficient than the vector containing wild type fiber via an CAR independent cell entry pathway.Conclusion: These results revealed that gene delivery by RGD-Adv was more efficient than that by conventional Adv and suggest that av-integrin-targeted Adv will be of great utility for gene therapy and gene transfer experiments.