LI Wei, et al. Study of the human amniotic epithelial cells on the fibrin clot and cellular growth factor[J]. Journal of Chongqing Medical University, 2006,(2):227-230.
LI Wei, et al. Study of the human amniotic epithelial cells on the fibrin clot and cellular growth factor[J]. Journal of Chongqing Medical University, 2006,(2):227-230.DOI:
Objective: To establish the model of cultured human amniotic epithelial cells(HAECs) on the fibrin clot in order to observe the effect of EGF
bFGF and TGF-β1 on HAEC.Methods:The cultivated HAECs on the formulated fibrin clot were examined by phase contrast microscopy
Giemsa staining and scanning electron microscopy.The effect of growth factor(EGF
bFGF
and TGF-β1) on the apoptosis of HAECs was measured by TUNEL.Results: HAECs generated on the formulated fibrin clot in vitro.HAECs exposed to EGF or bFGF showed a lower rate of apoptosis than the control group(P<0.05)
however
HAECs exposed to TGF-β1 showed an opposite effect(P<0.05).Conclusion: HAECs generate well on the formulated fibrin clot in vitro.Exposure to EGF or bFGF decreases the apoptosis rate of HAEC