Objective: To accomplish the detachment and culture of IPE in vitro and to investigate the course of the transdifferentiation from iris pigmented epithelial cell to lens epithelial cell during the specific condition.Methods: The IPE of rabbits were detached and cultivated according to the method of enzyme-assisted microdissection.Then the IPE were cultivated in the culture medium appending with PTU? Huase? rhFGF-4? Asc-2P and so on.Finally the initiative and the ultimate cells were respectively identified by microscope?electroscope and the method of immunohistochemistry.Results: The IPE of rabbits were successfully detached and cultivated and the course of the transdifferentiation was observed.The IPE and LEC were identified by their particular reaction to immunohistochemistry.Conclusion: It is a good method to detach IPE by enzyme-assisted microdissection.During in vitro culture

IPE can undergo the course of transdifferentiation and turn into LEC finally.This experiment suggest that those factors such as PTU? Huase? rhFGF-4? Asc-2P and so on may be involved the regulation of the course of transdifferentiation.