Object: To investigate effect of different Laboratory conditions to examination of some hemostasis: prothrombin time(PT)

activated partial thromboplastin time(APTT)

fibrinogen(FIB) and thrombin time(TT).Methods: After treated with different method by appropriate reagent

specimens were measured for hemostasis by Sysmex CA7000 automatic machine.In method one blood was collected by general anticoagulative glass tube without silicification..In method two blood was collected by specific anticoagulative silicification glass tube provided by different manufactory.The measured result were compared by statistics method.Results: There was significant difference between method one and two when measured value of PT

APTT and TT were concerned(P<0.01).If centrifuging(2500r/min) time was less than 15 minutes

the detected value of PT

APTT would be significantly longer than normal(P<0.01).If the blood sample was transferred to plastic cup before added to automatic machine

all the detected value would be significantly longer(P<0.01).If PT was examined in room temperature in 8 h

APTT in 4 h

FIB and TT in 24 h

there was no difference in the detected result compared with examined immediately after sampled.Conclusion: Specimens for hemostasis examination should be ampled with specific tube

centrifuged in adequate time

and should be detected in limited time.