LIU Guoliang, et al. Effect of salvia miltiorrhiza bunge on proliferation and differentiation of osteoblasts in rats[J]. Journal of Chongqing Medical University, 2006,(6):865-867.
LIU Guoliang, et al. Effect of salvia miltiorrhiza bunge on proliferation and differentiation of osteoblasts in rats[J]. Journal of Chongqing Medical University, 2006,(6):865-867.DOI:
Objective:To observe the effects of Salvia Miltiorrhiza Bunge on cell proliferation and differentiation of osteoblasts in rats cultured in vitro.Methods:Osteoblasts from cranium of newborn SD rats were cultured by collagenase method.MTT was used to determine the proliferation of cultured osteoblasts affected by different concentrations of Salvia Miltiorrhiza Bunge at different time points(24
48
72 hrs after medication).Differentiating effect of different concentrations of Salvia Miltiorrhiza Bunge in the cell was evaluated through the examination of alkali phosphate(ALP) activity.Results:0.05%
0.10%
0.15% of Salvia Miltiorrhiza Bunge could enhance the cell proliferation rate(P<0.05)
and different concentrations of Salvia Miltiorrhiza Bunge could enhance ALP activity(P<0.05).Conclusion:Salvia Miltiorrhiza Bunge could promote the proliferation and differentiation of the osteoblasts.