Objective:To clone and sequence the full-length of human PPARδ cDNA in order to further construct the eukaryotic expression vector carrying hPPARδ gene.Methods:hPPARδ cDNA was cloned from HepG2 cells total RNA by RT-PCR.The PCR product recovered from gel were ligated with pMD19-T vector and transformed into DH5α competent cell.The integrity and fidelity of hPPARδ cDNA sequence inserted in T vector were verified by BamH I and Sal I double excising and DNA sequencing assays.Results:The positive clone T vector plasmid containing correct sequence of hPPARδ cDNA were verified by enzyme digestion as well as sequence analysis and was named as pMD19-hPPARδ-T vector.The sequence of inserted hPPARδ cDNA was in accordance with the corresponding sequence in GeneBank database(AY919140).Conclusion:hPPARδ gene is successfully cloned and the pMD19-hPPARδ-T intermediate vector is constructed

which provide a good basis for further constructing the eukaryotic expression vector carrying hPPARδ gene and studying the receptor’s function.