Objective:To provide material foundation for the transplantation of neural stem cells(NSCs)in the treatment of cerebral ischemia

we transfected NSCs using recombinant adenovirus with HIF-1α and GFP gene

and observed the expression of HIF-1α and GFP

and their effects on bionomics of the NSCs.Methods:NSCs were transfected with the recombinant adenovirus

and then the expression of HIF-1α and GFP in the NSCs was observed.NSCs were detected under phase contrast microscope and fluorescent microscope.The special protein such as nestin

neurofilment protein(NFP)

glial fibriliary acidic protein(GFAP)were detected with immunofluorenscence technique.Results:The exogenous gene could be expressed steadily in NSCs after transfection.The morphological features of the untransfected NSCs were similar to those of the transfected

and there were no significant differences between the transfected NSCs and the untransfected in the cytoactivity and differentiation(P>0.05).The transfected NSCs were confirmed to have the latent ability of multi-directional differentiation.Conclusion:NSCs can express HIF-1α and GFP steadily after transfected with the recombinant adenovirus and the bionomics is normal.