YANG Qin, et al. Identification and cultivation of neural stem cells in neonatal rats in vitro[J]. Journal of Chongqing Medical University, 2007,(11):1134-1137.
YANG Qin, et al. Identification and cultivation of neural stem cells in neonatal rats in vitro[J]. Journal of Chongqing Medical University, 2007,(11):1134-1137.DOI:
Objective:To study the identification and cultivation of neural stem cells in neonatal rats in vitro.Methods:Cell culture in serum-free medium
indirect immunofluorescence cytochemistry were used.Results:The neural stem cells isolated in newborn rats had the potential of forming clones and serial passage
expressing neuroepithelial stem cell protein(nestin)and differentiating into neurons and astrocytes and oligodendrocytes.Conclusion:Neural stem cells maintain their undifferentiated features and have the capacity of self-renewal