X射线对肺腺癌细胞粘附分子及其相关生物学特性的影响

刘晓; 蒋幼凡; 岳秀; 雷淑慧

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X射线对肺腺癌细胞粘附分子及其相关生物学特性的影响

论著_基础研究 | 更新时间：2024-01-17

- X射线对肺腺癌细胞粘附分子及其相关生物学特性的影响
- The effect of X-irradiation on adhesion molecules and their related biological characteristics of A549 human lung adenocarcinoma cell line
- 重庆医科大学学报 2008年第1期页码：25-28
- 作者机构：
  
  1. 重庆医科大学附属第二医院呼吸内科
  2. 重庆医科大学附属第二医院呼吸内科,重庆,400010
  3. 重庆医科大学基础医学研究所
  4. ,重庆,400016
- 作者简介：
- 基金信息：
  
  重庆市卫生科技项目（03-2-053）
- DOI：
  中图分类号： R734.2
- 纸质出版日期：2008，
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[1]刘晓,蒋幼凡,岳秀,雷淑慧.X射线对肺腺癌细胞粘附分子及其相关生物学特性的影响[J].重庆医科大学学报,2008(01):25-28.

LIU Xiao, et al. The effect of X-irradiation on adhesion molecules and their related biological characteristics of A549 human lung adenocarcinoma cell line[J]. Journal of Chongqing Medical University, 2008,(1):25-28.
[1]刘晓,蒋幼凡,岳秀,雷淑慧.X射线对肺腺癌细胞粘附分子及其相关生物学特性的影响[J].重庆医科大学学报,2008(01):25-28. DOI：

LIU Xiao, et al. The effect of X-irradiation on adhesion molecules and their related biological characteristics of A549 human lung adenocarcinoma cell line[J]. Journal of Chongqing Medical University, 2008,(1):25-28. DOI：

摘要

目的:探讨X射线对肺腺癌细胞粘附分子及其相关生物学特性的影响。方法:不同剂量X射线照射肺腺癌细胞A549

MTT法检测细胞增殖抑制;免疫细胞化学法蛋白水平检测细胞中E-cadherin及其连环蛋白β-catenin的表达;Transwell小室检测细胞的侵袭力。结果:X射线对A549细胞的增殖抑制作用呈剂量依赖性;照射后E-cadherin和β-catenin蛋白的表达均显著高于对照组（P<0.05）

其变化趋势是随照射剂量增加表达逐渐增加;Transwell小室结果表明随着照射剂量的增加

细胞侵袭力逐渐降低（P<0.05）。结论:X射线上调A549细胞上粘附分子E-cadherin和β-catenin在细胞中的表达

增加细胞间的粘附力

降低肿瘤细胞的侵袭力

这可能是X射线抑制肺腺癌细胞转移的作用机制之一。

Abstract

Objective:To investigate the effect of X-irradiation on Adhesion molecules and their related biological characteristics of Human Lung.Methods:A549 cells were irradiated with varying doses of X-rays.MTT assay was employed to evaluate the effects of X-irradiation on the proliferative inhibition of A549 cells.The immunocytochemical method was employed to analyze the protein expression levels of E-cadherin and β-catenin.The transwell chambers were employed to evaluate the invasion of A549 cells.Results:X-irradiation could inhibit the proliferation of A549 cells in a dose-dependent manner.The protein expression of E-cadherin and β-catenin in A549 were higher than that in control group(P<0.05).And the cell invasions were lower than that in control group(P<0.05).The expression of E-cadherin

β-catenin and cell invasions showed a direct correlation with the dose.Conclusion:Up-regulating the expression of E-cadherin and β-catenin on A549 cells

which enhanced the adhesion between cells and depressed the invasion.That may be the mechanism of X-irradiation inhibiting the metastasis of Human Lung Adenocarcinoma Cell line.

关键词

X射线A549E-cadherinβ-catenin转移

Keywords

X-irradiationA549E-cadherinβ-cateninmetastasis

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