Objective:To explore the effect of heredity on functional states of Thl/Th2 in cord blood mononuclear cells(CBMC)of neonates

whether their parents have anaphylactic disease. Methods:Cord blood was taken from 40 normal full term neonatals

who were divided into 20 ones with their parents having anaphylactic disease(AD group)

and 20 not(control group).Collect blood plasma and cord blood mononuclear cells(CBMC)of neonates were separated

Blood plasma was collected and after stimulation with PHA

cell proliferation was detected by MTT method. IL-2

IL-4

IFN-r

and GM-CSF in supernatant were assayed by ELASA after CBMCs were cultured in vitro for 48h. Results:In AD group

as compared with control group:(1)The expression of IL-2

IL-4 and GM-CSF in blood plasma were higher than those of control group(P<0.05)

but not IFN-r.(2)Cytokines’ expression of IL-2

IL-4

IFN-r

and GM-CSF were increased after PHA stimulation for 48 hours

with significant different from those in control group(P<0.05). Conclusion:Heredity effect may play an important role in generation and progression of newborn allergic diseases.