Objective:To investigate the effects of anti-CXCR4 monoclonal antibody 12G5 on adhesion and invasion of SGC7901 cells.Methods:The effects of 12G5(5μg/ml

10μg/ml and 20μg/ml

respectively)on the adhesive、invasive and migrative abilities of SGC7901 cells were explored by MTT and Transwell chamber. Results:(1)12G5 showed a time/dose-dependent inhibition effect on SGC7901 cells adhering Matrigel

and the adhesion of gastric cancer cells was inhibited on the concentration of 10μg/ml of 12G5

and compared with untreated group

adhesion of SGC7901 cells was signficantly lower(P<0.01);(2)The invaded cell numbers in invasive and metastatic experiments of SGC7901 cell lines untreated or treated with 12G5 were 121.7±5.51

54.3±9.07;and 140.3±6.03

62.0±11.53 respectively

the invasive and metastatic abilities of treated group were lower than those of untreated group significantl(P<0.01). Conclusion:12G5 may inhibit adhesion and invasion of SGC7901 cells to certain extent.