Objective:To prove that haemodialysis patients sera medium can induce angiogenesis by oxidative stress. Method: Eleven haemodialysis patients were administered 400mg/d of vitamin E for 6 weeks per os (treated group)

and another eleven haemodialysis patients were treated regularly(non-treated group).HUVEC were cultured in 10% treated patients sera medium and 10% non-treated patients sera medium respectively. The plasma level of MDA was detected by TBA method. Angiogenesis were observed under inverted microscope

cell proliferation examinded by MTT

cell migration measured in transwell

and ROS investigated by colorimetry. Results:In treated group

the plasma level of MDA was lower after treatment.There were tube formation(average area as 1 438μm2±112μm2) and more cell proliferation and migration after cells were cultured in non- treated haemodialysis patients sera.The production of ROS was higher than that of cells exposed to treated patients sera medium.Conclusion:Haemodialysis patients sera medium can induce angiogenesis and oxidative stress is the important mechanism.