TIAN Jing, et al. The biological character and fluorescent labeling of the fetal bone mesenchymal stem cells[J]. Journal of Chongqing Medical University, 2008,(6):665-668.
TIAN Jing, et al. The biological character and fluorescent labeling of the fetal bone mesenchymal stem cells[J]. Journal of Chongqing Medical University, 2008,(6):665-668.DOI：
proliferation and fluor-labelling of the fetal bone mesenchymal stem cell (BMSCs)
and their biological character. Methods:Limb bone marrow samples from fetus aged 4 to 5 months were obtained
BMSCs were isolated by Ficoll centrifugalization and adherence screening method
and then cultured
proliferated to the 12th generation in vitro. The growth curve
cell cycle
phenotype
enzymatic activity
glycogen and the DAPI fluor-labelling of BMSCs were observed. Results:(1)Major BMSCs were fusiform
arranged as whirlpool. After the 12th generation
BMSCs presented the signs of senility. The subcultured cells proliferated faster than primary cells. (2)The 3rd and 8th generation of BMSCs expressed CD29
D71
but not CD34
HLA-DR;the G0
G1 period cells were 93.73%
and the S
M
G2 period cells were 6.27%. (3)The 5th generation cells were positive of nonspecific esterase and glycogen
but weak positive of alkaline phosphatase. (4)DAPI labeled BMSCs grew normaly
and the marking rate of BMSCs was 100%
but decreased gradually
and only 15% cells were labbelled for the 3th week. Conclusion:The acquired fetal BMSCs have the character of MSCs. The DAPI labeling is a modus operandi for the short period tracing BMSCs.
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