Objective:To study the change of the survivin mRNA and protein of BEP-2D cells during its malignant transformation.Methods:Normal BEP-2D cell and BEP-2D cells treated by cigarette smoke condensate(CSC)for 15 weeks(P-15)

25 weeks(P-25)and 38 weeks(P-38)were respectively chosen to study the survivin gene and protein by RT-PCR(retro-translation PCR

RT-PCR)and immunohistochemical method.Results:The survivin mRNA was found in BEP-2D cells of P-15

P-25 and P-38

respectively with 0.56

0.80

and 0.81

but not in the normal BEP-2D cell.The survivin protein was found in the normal BEP-2D cell

but not in BEP-2D cells of P-15

P-25 and P-38.The levels of survivin protein expressian were different between BEP-2D cells of P-15

P-25 and P-38 and normal BEP-2D cell

with significant difference between P-15 BEP-2D cell and normal BEP-2D cell(P<0.05).Conclusion:The survivin mRNA and protein may be consider of a kind of biology-marker for the early diagnosis of lung cancer because the survivin mRNA and protein occur in the earlier period of lung cancer.